Transcript: Mouse NM_008217.4

Mus musculus hyaluronan synthase 3 (Has3), transcript variant 1, mRNA.

Source:
NCBI, updated 2017-05-28
Taxon:
Mus musculus (mouse)
Gene:
Has3 (15118)
Length:
5912
CDS:
174..1838

Additional Resources:

NCBI RefSeq record:
NM_008217.4
NBCI Gene record:
Has3 (15118)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_008217.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000076265 CGTGAACTTCATTGGCCTAAT pLKO.1 1601 CDS 100% 10.800 15.120 N Has3 n/a
2 TRCN0000418844 GATTAGCCATCAATGCGTTAA pLKO_005 2294 3UTR 100% 10.800 15.120 N Has3 n/a
3 TRCN0000076263 CCGATCTGAAACGCAAAGAAT pLKO.1 1948 3UTR 100% 5.625 7.875 N Has3 n/a
4 TRCN0000076267 GCAAGTCTTACTTTCGGGAAT pLKO.1 1249 CDS 100% 4.050 5.670 N Has3 n/a
5 TRCN0000076264 GCAATGTATTAGTGGGCCTTT pLKO.1 1016 CDS 100% 4.050 5.670 N Has3 n/a
6 TRCN0000417066 TTGGCTACCGGACTAAGTATA pLKO_005 1156 CDS 100% 13.200 9.240 N Has3 n/a
7 TRCN0000076266 CCAGGAAGATACCTACATGTT pLKO.1 554 CDS 100% 4.950 3.465 N Has3 n/a
8 TRCN0000415836 TGCTGTATCTGGCCATTATTG pLKO_005 1762 CDS 100% 13.200 7.920 N Has3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_008217.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_06352 pDONR223 100% 43% 42.8% None (many diffs) n/a
2 ccsbBroad304_06352 pLX_304 0% 43% 42.8% V5 (many diffs) n/a
3 TRCN0000481493 AGATTCATTAACACTGCTTTCTAT pLX_317 44.2% 43% 42.8% V5 (many diffs) n/a
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