Transcript: Mouse NM_009601.4

Mus musculus cholinergic receptor, nicotinic, beta polypeptide 1 (muscle) (Chrnb1), mRNA.

Source:
NCBI, updated 2017-05-20
Taxon:
Mus musculus (mouse)
Gene:
Chrnb1 (11443)
Length:
2151
CDS:
59..1564

Additional Resources:

NCBI RefSeq record:
NM_009601.4
NBCI Gene record:
Chrnb1 (11443)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_009601.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000351101 GCCGAAGGCCAACTGATTAAG pLKO_005 134 CDS 100% 13.200 18.480 N Chrnb1 n/a
2 TRCN0000339725 ATAGCGCTTGGCGCTTGTAAG pLKO_005 1613 3UTR 100% 10.800 15.120 N Chrnb1 n/a
3 TRCN0000339654 CCTAGATGCCACGTACCATTT pLKO_005 1519 CDS 100% 10.800 15.120 N Chrnb1 n/a
4 TRCN0000339652 GTGGACCGACTACAGGTTAAG pLKO_005 304 CDS 100% 10.800 15.120 N Chrnb1 n/a
5 TRCN0000102971 GCTGAACAACAATGACGGAAA pLKO.1 403 CDS 100% 4.050 5.670 N Chrnb1 n/a
6 TRCN0000339726 TGAACAACAATGACGGAAATT pLKO_005 405 CDS 100% 13.200 9.240 N Chrnb1 n/a
7 TRCN0000102973 CAATGGGAGATTATCCACAAA pLKO.1 677 CDS 100% 4.950 3.465 N Chrnb1 n/a
8 TRCN0000102970 CCCAACAATGTTACTTCCTTA pLKO.1 1829 3UTR 100% 4.950 3.465 N Chrnb1 n/a
9 TRCN0000102972 CCTTCTAGGTTAATCCAACTT pLKO.1 698 CDS 100% 4.950 3.465 N Chrnb1 n/a
10 TRCN0000102974 CTGAACCACATCACTCTCTTT pLKO.1 1170 CDS 100% 4.950 2.970 N Chrnb1 n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_009601.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00308 pDONR223 100% 85.6% 89.8% None (many diffs) n/a
2 ccsbBroad304_00308 pLX_304 0% 85.6% 89.8% V5 (many diffs) n/a
3 TRCN0000479543 TTCAGGGCTAATCTCCAACTCGAC pLX_317 23.5% 85.6% 89.8% V5 (many diffs) n/a
Download CSV