Construct: ORF TRCN0000479543
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003640.1_s317c1
- Derived from:
- ccsbBroadEn_00308
- DNA Barcode:
- TTCAGGGCTAATCTCCAACTCGAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CHRNB1 (1140)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479543
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1140 | CHRNB1 | cholinergic receptor nicoti... | NM_000747.3 | 100% | 100% | |
| 2 | mouse | 11443 | Chrnb1 | cholinergic receptor, nicot... | NM_009601.4 | 85.6% | 89.8% | (many diffs) |
| 3 | mouse | 11443 | Chrnb1 | cholinergic receptor, nicot... | XM_006532003.2 | 82.7% | 86.8% | (many diffs) |
| 4 | mouse | 11443 | Chrnb1 | cholinergic receptor, nicot... | XM_006532004.3 | 73.9% | 78% | (many diffs) |
| 5 | mouse | 11443 | Chrnb1 | cholinergic receptor, nicot... | XM_006532005.3 | 50.2% | 48.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1569
- ORF length:
- 1503
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac cccaggggct ctgctgatgc tgctgggggc gctgggggcg ccgctcgccc 121 caggcgtccg cggctcggag gcggagggtc gactccggga gaaacttttc tctggctatg 181 atagctccgt gcggccagcg cgggaggtgg gagaccgtgt cagggtcagc gttggtctca 241 tcctggcgca actcatcagc ctgaacgaga aggatgaaga gatgagcaca aaggtgtact 301 tagacctgga gtggactgac tacaggctga gctgggaccc tgcggagcac gacggcatcg 361 attcgctccg catcacggcg gaatccgtgt ggctccctga cgtggtgcta ctgaacaaca 421 atgatgggaa ttttgacgtg gctctggaca ttagcgtcgt ggtgtcctcc gacggctccg 481 tgcgttggca acccccgggc atctatcgca gcagctgcag catccaggtc acctacttcc 541 ccttcgactg gcagaattgc actatggtgt tcagctccta cagctacgac agctcggagg 601 tcagcctgca gacaggcctg ggtcctgacg ggcaagggca tcaggaaatc cacattcatg 661 aagggacttt cattgagaat ggccagtggg agattatcca caagccctct cggctaatcc 721 agcctccagg cgatcctagg ggagggaggg aaggacagcg ccaggaagtc atcttctacc 781 tcatcatccg ccgcaagcct ctcttctacc tggtcaacgt cattgcccca tgcatcctca 841 tcactcttct ggccatcttc gtcttctacc tgccaccaga tgcaggagag aagatggggc 901 tctcaatctt tgccctgctg acccttactg tgttcctgct gctgctggct gacaaagtac 961 ctgagacctc actatcagta cccattatta tcaagtacct catgtttacc atggtcctcg 1021 tcaccttctc agtcatcctt agtgtcgtgg ttctcaacct gcaccaccgc tcaccccaca 1081 cccaccaaat gcccctttgg gtccgtcaga tcttcattca caaacttccg ctgtacctgc 1141 gtctaaaaag gcccaaaccc gagagagacc tgatgccgga gccccctcac tgttcttctc 1201 caggaagtgg ctggggtcgg GGAACAGATG AATATTTCAT CCGGAAGCCG CCAAGTGATT 1261 TTCTCTTCCC CAAACCCAAT AGGTTCCAGC CTGAACTGTC TGCCCCTGAT CTGCGGCGAT 1321 TTATCGATGG TCCAAACCGG GCTGTGGCCC TGCTTCCGGA GCTACGGGAG GTCGTCTCCT 1381 CTATCAGCTA CATCGCTCGA CAGCTGCAGG AACAGGAGGA CCACGATGCG CTGAAGGAGG 1441 ACTGGCAGTT TGTGGCCATG GTAGTGGACC GCCTCTTCCT GTGGACTTTC ATCATCTTCA 1501 CCAGCGTTGG GACCCTAGTC ATCTTCCTGG ACGCCACGTA CCACTTGCCC CCTCCAGACC 1561 CCTTTCCTTG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1621 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1681 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGATTCAGG GCTAATCTCC AACTCGACAC 1741 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt