Transcript: Mouse NM_010212.4

Mus musculus four and a half LIM domains 2 (Fhl2), transcript variant 1, mRNA.

Source:
NCBI, updated 2017-06-03
Taxon:
Mus musculus (mouse)
Gene:
Fhl2 (14200)
Length:
1566
CDS:
284..1123

Additional Resources:

NCBI RefSeq record:
NM_010212.4
NBCI Gene record:
Fhl2 (14200)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_010212.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000113501 CGGCAAGAAGTACATTCTAAA pLKO.1 328 CDS 100% 13.200 18.480 N Fhl2 n/a
2 TRCN0000306221 TGGCACAACGACTGCTTTAAC pLKO_005 1013 CDS 100% 13.200 18.480 N Fhl2 n/a
3 TRCN0000113503 CTGTGACTTGTACGCTAAGAA pLKO.1 922 CDS 100% 5.625 7.875 N Fhl2 n/a
4 TRCN0000354009 CTGTGACTTGTACGCTAAGAA pLKO_005 922 CDS 100% 5.625 7.875 N Fhl2 n/a
5 TRCN0000306239 TATCACCACAGGAGGTGTTAC pLKO_005 787 CDS 100% 10.800 8.640 N Fhl2 n/a
6 TRCN0000306223 TTGAGGAACTCTATGCCAATA pLKO_005 378 CDS 100% 10.800 7.560 N Fhl2 n/a
7 TRCN0000005773 CGACTGCTTTAACTGTAAGAA pLKO.1 1021 CDS 100% 5.625 3.938 N FHL2 n/a
8 TRCN0000273528 CGACTGCTTTAACTGTAAGAA pLKO_005 1021 CDS 100% 5.625 3.938 N FHL2 n/a
9 TRCN0000113504 CCTCACAGAGAGAGATGACAT pLKO.1 1072 CDS 100% 4.950 3.465 N Fhl2 n/a
10 TRCN0000113500 GATGGGAAGATGGTTTGGAAT pLKO.1 1309 3UTR 100% 4.950 3.465 N Fhl2 n/a
11 TRCN0000326116 GATGGGAAGATGGTTTGGAAT pLKO_005 1309 3UTR 100% 4.950 3.465 N Fhl2 n/a
12 TRCN0000113502 CGACTGCTATTCCAATGAATA pLKO.1 553 CDS 100% 1.320 0.924 N Fhl2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_010212.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00568 pDONR223 100% 87.2% 92.1% None (many diffs) n/a
2 ccsbBroad304_00568 pLX_304 0% 87.2% 92.1% V5 (many diffs) n/a
3 TRCN0000466342 ATGTCCCGGCCCCCCTTAATCACA pLX_317 45.7% 87.2% 92.1% V5 (many diffs) n/a
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