Transcript: Human NM_012074.5

Homo sapiens double PHD fingers 3 (DPF3), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-09-30
Taxon:
Homo sapiens (human)
Gene:
DPF3 (8110)
Length:
4055
CDS:
14..1087

Additional Resources:

NCBI RefSeq record:
NM_012074.5
NBCI Gene record:
DPF3 (8110)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_012074.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000235748 GGTTCGCCCACAGCAGATAAA pLKO_005 1052 CDS 100% 13.200 18.480 N DPF3 n/a
2 TRCN0000013154 AGAGGATATTCCCAAGCGAAA pLKO.1 499 CDS 100% 4.050 5.670 N DPF3 n/a
3 TRCN0000013156 CCGGAGTTACAACTCACGGCT pLKO.1 85 CDS 100% 0.220 0.308 N DPF3 n/a
4 TRCN0000235746 ACGTACCACGGAGGACTTATT pLKO_005 934 CDS 100% 13.200 9.240 N DPF3 n/a
5 TRCN0000433553 ACGTACCACGGAGGACTTATT pLKO_005 934 CDS 100% 13.200 9.240 N Dpf3 n/a
6 TRCN0000086345 CGGCTTTGATGAGGACGATTT pLKO.1 991 CDS 100% 10.800 7.560 N Dpf3 n/a
7 TRCN0000235749 GGCTTTGATGAGGACGATTTG pLKO_005 992 CDS 100% 10.800 7.560 N DPF3 n/a
8 TRCN0000235747 TGGCGCAAGAAGAGACGATTG pLKO_005 248 CDS 100% 6.000 4.200 N DPF3 n/a
9 TRCN0000419432 CATCTGTGGCAAGCGCTACAA pLKO_005 616 CDS 100% 4.950 3.465 N Dpf3 n/a
10 TRCN0000013157 CTGGCGCAAGAAGAGACGATT pLKO.1 247 CDS 100% 4.950 3.465 N DPF3 n/a
11 TRCN0000235750 GGCATCGTGATGCGCTATTTA pLKO_005 1335 3UTR 100% 15.000 9.000 N DPF3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_012074.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07202 pDONR223 100% 99.7% 99.4% None 253T>C;530G>A;977A>G n/a
2 TRCN0000473387 GCCCTCCGGGGAGACATTCACATG pLX_317 40.7% 99.7% 99.4% V5 253T>C;530G>A;977A>G n/a
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