Construct: ORF TRCN0000473387
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003289.1_s317c1
- Derived from:
- ccsbBroadEn_07202
- DNA Barcode:
- GCCCTCCGGGGAGACATTCACATG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DPF3 (8110)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473387
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8110 | DPF3 | double PHD fingers 3 | NM_012074.5 | 99.7% | 99.4% | 253T>C;530G>A;977A>G |
| 2 | human | 8110 | DPF3 | double PHD fingers 3 | NM_001280543.1 | 95.4% | 94.2% | (many diffs) |
| 3 | human | 8110 | DPF3 | double PHD fingers 3 | NM_001280544.1 | 84.9% | 82.5% | (many diffs) |
| 4 | human | 8110 | DPF3 | double PHD fingers 3 | NM_001280542.1 | 84.3% | 77.6% | (many diffs) |
| 5 | mouse | 70127 | Dpf3 | D4, zinc and double PHD fin... | NM_001267626.1 | 92.9% | 99.1% | (many diffs) |
| 6 | mouse | 70127 | Dpf3 | D4, zinc and double PHD fin... | NM_058212.2 | 92.6% | 98.8% | (many diffs) |
| 7 | mouse | 70127 | Dpf3 | D4, zinc and double PHD fin... | NM_001267625.1 | 76.8% | 77.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1137
- ORF length:
- 1071
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gactgtcatt cacaaccccc tgaaagcgct cggggaccag ttctacaagg 121 aagccattga gcactgccgg agttacaact cacggctgtg tgcagagcgc agcgtgcgtc 181 ttcccttcct ggactcacag actggggtgg cccagaacaa ctgctacatc tggatggaga 241 agaggcaccg aggcccaggc cttgccccgg gccagctgta tacataccct gcccgctgct 301 ggcgcaagaa gagacgactg cacccacctg aagatccaaa actgcggctg ctggagataa 361 aacctgaagt ggagcttccc ctgaagaagg atgggttcac ctcagagagc accacgctgg 421 aagccttgct ccgtggcgag ggggttgaga agaaggtgga tgccagggag gaggaaagca 481 tccaggaaat acagagggtt ttggaaaatg atgaaaatgt agaagaaggg aatgaagaag 541 aggatttgga agaggatatt cccaagcgaa agaacaggac tagaggacgg gctcacggct 601 ctgcaggggg caggaggagg cacgacgccg cctctcagga agaccacgac aaaccttacg 661 tctgtgacat ctgtggcaag cgctacaaga accgaccggg gcTCAGCTAC CACTATGCTC 721 ACACTCACCT GGCCAGCGAG GAGGGGGATG AAGCTCAAGA CCAGGAGACT CGGTCCCCAC 781 CCAACCACAG AAATGAGAAC CACAGGCCCC AGAAAGGACC GGATGGAACA GTCATTCCCA 841 ATAACTACTG TGACTTCTGC TTGGGGGGCT CCAACATGAA CAAGAAGAGT GGGCGGCCTG 901 AAGAGCTGGT GTCCTGCGCA GACTGTGGAC GCTCTGCTCA TTTGGGAGGA GAAGGCAGGA 961 AGGAGAAGGA GGCAGCGGCC GCAGCACGTA CCACGGAGGA CTTATTCGGT TCCACGTCAG 1021 AAAGTGACAC GTCAACTTTC CGCGGCTTTG ATGAGGACGA TTTGGAAGAG CCTCGCTCCT 1081 GTCGAGGACG CCGCAGTGGC CGGGGTTCGC CCACAGCAGA TAAAAAGGGC AGTTGCTACC 1141 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1201 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1261 ATATATCTTG TGGAAAGGAC GAGCCCTCCG GGGAGACATT CACATGACGC GTTAAGTCga 1321 caatcaacct ctggattaca aaatttgtga aagatt