Transcript: Human NM_014970.4

Homo sapiens kinesin associated protein 3 (KIFAP3), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-08-22
Taxon:
Homo sapiens (human)
Gene:
KIFAP3 (22920)
Length:
2954
CDS:
229..2607

Additional Resources:

NCBI RefSeq record:
NM_014970.4
NBCI Gene record:
KIFAP3 (22920)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_014970.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000432050 GGACTTATTACTCACTATAAA pLKO_005 862 CDS 100% 15.000 12.000 N KIFAP3 n/a
2 TRCN0000432923 TTGATGAAGTTGCTAACATTA pLKO_005 605 CDS 100% 13.200 9.240 N KIFAP3 n/a
3 TRCN0000117241 CCAAACCTTGAGAAAGGATTA pLKO.1 990 CDS 100% 10.800 7.560 N KIFAP3 n/a
4 TRCN0000117237 CCCTCTGTCTTCTGTTAAGTA pLKO.1 2762 3UTR 100% 5.625 3.938 N KIFAP3 n/a
5 TRCN0000117240 CCTGATAACTTGGAAGAACTA pLKO.1 715 CDS 100% 4.950 3.465 N KIFAP3 n/a
6 TRCN0000117239 CCAGCATATCTCATAGACCTA pLKO.1 2131 CDS 100% 2.640 1.848 N KIFAP3 n/a
7 TRCN0000117238 GCCGTGATTCATTGTCAGGAA pLKO.1 524 CDS 100% 2.640 1.848 N KIFAP3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_014970.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07818 pDONR223 100% 99.9% 100% None 1065T>C n/a
2 ccsbBroad304_07818 pLX_304 0% 99.9% 100% V5 1065T>C n/a
3 TRCN0000469096 TACAAGACCATCACGAGGCATGGC pLX_317 14% 99.9% 100% V5 1065T>C n/a
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