Transcript: Human NM_015397.4

Homo sapiens DDB1 and CUL4 associated factor 12 (DCAF12), mRNA.

Source:
NCBI, updated 2019-05-02
Taxon:
Homo sapiens (human)
Gene:
DCAF12 (25853)
Length:
3592
CDS:
268..1629

Additional Resources:

NCBI RefSeq record:
NM_015397.4
NBCI Gene record:
DCAF12 (25853)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_015397.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000168140 CCTGTGAAGAGATCCTTAGTA pLKO.1 388 CDS 100% 5.625 3.938 N DCAF12 n/a
2 TRCN0000168680 GCTGGCTGAATCATGATGAAA pLKO.1 1463 CDS 100% 5.625 3.938 N DCAF12 n/a
3 TRCN0000168231 CATGATGAAACCTGGAGGAAT pLKO.1 1474 CDS 100% 4.950 3.465 N DCAF12 n/a
4 TRCN0000173036 CCTCCCTTGAACTGTCTGTTT pLKO.1 2904 3UTR 100% 4.950 2.970 N DCAF12 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_015397.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07953 pDONR223 100% 99.9% 99.7% None 392G>A n/a
2 ccsbBroad304_07953 pLX_304 0% 99.9% 99.7% V5 392G>A n/a
3 TRCN0000476003 TTATGAACTCCACTGCAACGATCG pLX_317 23.5% 99.9% 99.7% V5 392G>A n/a
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