Construct: ORF TRCN0000476003
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004958.1_s317c1
- Derived from:
- ccsbBroadEn_07953
- DNA Barcode:
- TTATGAACTCCACTGCAACGATCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DCAF12 (25853)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476003
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 25853 | DCAF12 | DDB1 and CUL4 associated fa... | NM_015397.4 | 99.9% | 99.7% | 392G>A |
| 2 | human | 25853 | DCAF12 | DDB1 and CUL4 associated fa... | XM_011517843.1 | 56.7% | 56.7% | 0_1ins588 |
| 3 | mouse | 68970 | Dcaf12 | DDB1 and CUL4 associated fa... | NM_026893.3 | 91.5% | 98.2% | (many diffs) |
| 4 | mouse | 68970 | Dcaf12 | DDB1 and CUL4 associated fa... | XM_006538228.3 | 88.9% | 94.4% | (many diffs) |
| 5 | mouse | 68970 | Dcaf12 | DDB1 and CUL4 associated fa... | XM_006538229.3 | 86.5% | 92.9% | (many diffs) |
| 6 | mouse | 68970 | Dcaf12 | DDB1 and CUL4 associated fa... | XM_006538230.3 | 52.1% | 56.2% | (many diffs) |
| 7 | mouse | 68970 | Dcaf12 | DDB1 and CUL4 associated fa... | XM_017320381.1 | 52.1% | 56.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1428
- ORF length:
- 1359
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcccggaaa gtagttagca ggaagcggaa agcgcccgcc tcgccgggag 121 ctgggagcga cgctcagggc ccgcagtttg gctgggatca ctcgcttcac aaaaggaaaa 181 gacttcctcc tgtgaagaga tccttagtat actacttgaa gaaccgggaa gtcaggctac 241 agaatgaaac cagctactct cgagtgttgc atggttatgc agcacagcaa cttcccagtc 301 tcctgaagga gagagagttt caccttggga cccttaataa agtgtttgca tctcagtggt 361 tgaatcatag gcaagtggtg tgtggcacaa aatgcaacac gctatttgtc gtagatgtcc 421 agacaagcca gatcaccaag atccccattc tgaaagacca ggagcctgga ggtgtgaccc 481 agcagggctg tggtatccat gccatcgagc tgaatccttc tagaacactg ctagccactg 541 gaggagacaa ccccaacagt cttgccatct atcgactacc tacgctggat cctgtgtgtg 601 taggagatga tggacacaag gactggatct tttccatcgc atggatcagc gacactatgg 661 cagtgtctgg ctcacgtgat ggttctatgg gactctggga ggtgacagat gatgttttga 721 ccaaaagtga tgcgagacac aatgtgtcac gggtccctgt gtatgcacac atcactcaca 781 aggccttaaa ggacatcccc aaagaagaca caaaccctga caactgcaag gttcgggctc 841 tggccttcaa caacaagaac aaggaactgg gagcagtgtc tctggatggc tactttcatc 901 tctggaaggc tgaaaataca ctatctaagc tcctctccac caaactgcca tattgccgtg 961 agaatgtgtg tctggcttat ggtagtgaat ggtcagttta tgcagtgggc tcccaagctc 1021 atgtctcctt cttggatcca cggcagccat catacaacgt caagtctgtc tgttccaggg 1081 agcgaggcag tggaatccgg tcagtgagtt tcTACGAGCA CATCATCACT GTGGGAACAG 1141 GGCAGGGCTC CCTGCTGTTC TATGACATCC GAGCTCAGAG ATTTCTGGAA GAGAGGCTCT 1201 CAGCTTGTTA TGGGTCCAAG CCCAGACTAG CAGGGGAGAA TCTGAAACTA ACCACTGGCA 1261 AAGGCTGGCT GAATCATGAT GAAACCTGGA GGAATTACTT TTCAGACATT GACTTCTTCC 1321 CCAATGCTGT TTACACCCAC TGCTACGACT CGTCTGGAAC GAAACTCTTT GTGGCAGGAG 1381 GTCCCCTCCC TTCAGGGCTC CATGGAAACT ATGCTGGGCT CTGGAGTTTG CCAACTTTCT 1441 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1501 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1561 GTGGAAAGGA CGATTATGAA CTCCACTGCA ACGATCGACG CGTTAAGTCg acaatcaacc 1621 tctggattac aaaatttgtg aaagatt