Transcript: Mouse NM_015752.3

Mus musculus suppressor of fused homolog (Drosophila) (Sufu), transcript variant 1, mRNA.

Source:
NCBI, updated 2017-06-03
Taxon:
Mus musculus (mouse)
Gene:
Sufu (24069)
Length:
4525
CDS:
208..1665

Additional Resources:

NCBI RefSeq record:
NM_015752.3
NBCI Gene record:
Sufu (24069)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_015752.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000089056 CGTTTCGTCTGAAGAGAGAAA pLKO.1 569 CDS 100% 4.950 6.930 N Sufu n/a
2 TRCN0000324975 CGTTTCGTCTGAAGAGAGAAA pLKO_005 569 CDS 100% 4.950 6.930 N Sufu n/a
3 TRCN0000089053 CCTGAACTTTATTGTCCTTTA pLKO.1 2167 3UTR 100% 10.800 7.560 N Sufu n/a
4 TRCN0000019467 CTTTGGATAACAGTGAGTCAA pLKO.1 704 CDS 100% 4.950 3.465 N SUFU n/a
5 TRCN0000089054 GCACTTCACCTACAAGAGTAT pLKO.1 1389 CDS 100% 4.950 3.465 N Sufu n/a
6 TRCN0000353956 GCACTTCACCTACAAGAGTAT pLKO_005 1389 CDS 100% 4.950 3.465 N Sufu n/a
7 TRCN0000089055 GCATTGAGACAGACGGTTCTA pLKO.1 983 CDS 100% 4.950 3.465 N Sufu n/a
8 TRCN0000325046 GCATTGAGACAGACGGTTCTA pLKO_005 983 CDS 100% 4.950 3.465 N Sufu n/a
9 TRCN0000089057 GAGGAATTTAAACTTCCCAAA pLKO.1 1570 CDS 100% 4.050 2.835 N Sufu n/a
10 TRCN0000325044 GAGGAATTTAAACTTCCCAAA pLKO_005 1570 CDS 100% 4.050 2.835 N Sufu n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_015752.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_15072 pDONR223 97.7% 90.9% 97.5% None (many diffs) n/a
2 ccsbBroad304_15072 pLX_304 0% 90.9% 97.5% V5 (many diffs) n/a
3 TRCN0000478839 GGCTGTTAAAGTGCGCTGAATCTT pLX_317 23.9% 90.9% 97.5% V5 (many diffs) n/a
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