Transcript: Mouse NM_019417.3

Mus musculus PDZ and LIM domain 4 (Pdlim4), mRNA.

Source:
NCBI, updated 2019-09-21
Taxon:
Mus musculus (mouse)
Gene:
Pdlim4 (30794)
Length:
1189
CDS:
86..1078

Additional Resources:

NCBI RefSeq record:
NM_019417.3
NBCI Gene record:
Pdlim4 (30794)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_019417.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000075388 GAACGGCTGTACTGTGAGAAT pLKO.1 977 CDS 100% 4.950 3.465 N Pdlim4 n/a
2 TRCN0000075391 TGAACGGCTGTACTGTGAGAA pLKO.1 976 CDS 100% 4.950 3.465 N Pdlim4 n/a
3 TRCN0000075390 CAAGGCTCAAGCACATAGGAT pLKO.1 367 CDS 100% 3.000 2.100 N Pdlim4 n/a
4 TRCN0000075392 GCGAGAGACAAGCTCTACCAT pLKO.1 890 CDS 100% 3.000 2.100 N Pdlim4 n/a
5 TRCN0000075389 AGGTGACTTGATCCAAGCCAT pLKO.1 220 CDS 100% 2.640 1.848 N Pdlim4 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_019417.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07267 pDONR223 100% 85.3% 90.9% None (many diffs) n/a
2 ccsbBroad304_07267 pLX_304 0% 85.3% 90.9% V5 (many diffs) n/a
3 TRCN0000470310 TGTTTCAGGTTTCAACTTGTGCCA pLX_317 34.5% 85.3% 90.9% V5 (many diffs) n/a
4 ccsbBroadEn_15639 pDONR223 0% 63.6% 61.4% None (many diffs) n/a
5 ccsbBroad304_15639 pLX_304 0% 63.6% 61.4% V5 (many diffs) n/a
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