Transcript: Human NM_024819.7

Homo sapiens dephospho-CoA kinase domain containing (DCAKD), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-06-01
Taxon:
Homo sapiens (human)
Gene:
DCAKD (79877)
Length:
2143
CDS:
359..1054

Additional Resources:

NCBI RefSeq record:
NM_024819.7
NBCI Gene record:
DCAKD (79877)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001144969 GGATATCCAGAATCACGTAG pXPR_003 CGG 326 47% 4 0.8183 DCAKD DCAKD 75872
2 BRDN0001148499 CACTCACCAGTATACTACCA pXPR_003 CGG 394 57% 4 0.808 DCAKD DCAKD 75873
3 BRDN0001148979 GGGCTGTGCGGTGATTGACG pXPR_003 TGG 88 13% 2 0.4946 DCAKD DCAKD 75874
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_024819.7, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000377648 CAAGGGTTTCCATGTTCAAAC pLKO_005 1517 3UTR 100% 10.800 15.120 N DCAKD n/a
2 TRCN0000380223 AGGGTTCACTGGGCTTGAATC pLKO_005 1430 3UTR 100% 10.800 8.640 N DCAKD n/a
3 TRCN0000360299 CTTTGGAGTGTATCCTATTTG pLKO_005 1246 3UTR 100% 13.200 9.240 N DCAKD n/a
4 TRCN0000360300 GAAGCACACCGTGGTAGTATA pLKO_005 739 CDS 100% 13.200 9.240 N DCAKD n/a
5 TRCN0000382037 TGCTGGAGAACGGCGACATAA pLKO_005 531 CDS 100% 13.200 9.240 N DCAKD n/a
6 TRCN0000078714 CCGCTACGTGATTCTGGATAT pLKO.1 679 CDS 100% 10.800 7.560 N DCAKD n/a
7 TRCN0000078717 CCTGCTGTTTGAGACCAAGAA pLKO.1 703 CDS 100% 4.950 3.465 N DCAKD n/a
8 TRCN0000078713 GCCAGGTAACACATCCTGTTT pLKO.1 1110 3UTR 100% 4.950 3.465 N DCAKD n/a
9 TRCN0000166364 CACACACACACACACACACAA pLKO.1 1148 3UTR 100% 4.950 2.475 Y KAAG1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_024819.7, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04143 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_04143 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000468920 ACGAGTGCAACTTTACATCTACAT pLX_317 50.2% 100% 100% V5 n/a
4 ccsbBroadEn_15156 pDONR223 0% 100% 100% None n/a
5 ccsbBroad304_15156 pLX_304 0% 100% 100% V5 n/a
6 TRCN0000465914 ACAGGCTATCTCGGTAAAGTGATA pLX_317 35.9% 100% 100% V5 n/a
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