Construct: ORF TRCN0000468920

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF009863.3_s317c1
Derived from:
ccsbBroadEn_04143
DNA Barcode:
ACGAGTGCAACTTTACATCTACAT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
DCAKD (79877)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000468920
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 79877 DCAKD dephospho-CoA kinase domain... NM_001128631.3 100% 100%
2 human 79877 DCAKD dephospho-CoA kinase domain... NM_001288654.2 100% 100%
3 human 79877 DCAKD dephospho-CoA kinase domain... NM_001288655.2 100% 100%
4 human 79877 DCAKD dephospho-CoA kinase domain... NM_001321326.2 100% 100%
5 human 79877 DCAKD dephospho-CoA kinase domain... NM_024819.7 100% 100%
6 human 79877 DCAKD dephospho-CoA kinase domain... XM_011525262.2 100% 100%
7 human 79877 DCAKD dephospho-CoA kinase domain... XM_017025105.1 55.2% 54.3% 0_1ins285;5_8delGCCC;13_13delAins23
8 human 79877 DCAKD dephospho-CoA kinase domain... XM_005257688.2 51.1% 48.9% (many diffs)
9 human 79877 DCAKD dephospho-CoA kinase domain... XM_017025103.1 51.1% 48.9% (many diffs)
10 human 79877 DCAKD dephospho-CoA kinase domain... XM_017025104.1 51.1% 48.9% (many diffs)
11 human 79877 DCAKD dephospho-CoA kinase domain... XR_002958070.1 47.2% 1_292del;609_716del;916_917ins177
12 mouse 68087 Dcakd dephospho-CoA kinase domain... NM_026551.3 86% 90.9% (many diffs)
13 mouse 68087 Dcakd dephospho-CoA kinase domain... XM_006534038.2 86% 90.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
759
ORF length:
693
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgtt tctggtgggc ctgacagggg gcattgcctc aggcaagagc tcagtgatcc
121aggtgttcca gcagctgggc tgtgcggtga ttgacgtgga cgtgatggcc cggcacgtcg
181tgcagccagg ataccctgcc caccggcgca tcgtagaggt cttcggcact gaggtcttgc
241tggagaacgg cgacataaat cgcaaggtcc tgggggacct gatctttaac cagcctgacc
301ggcggcagct gctcaacgcc atcacccacc ccgagattcg caaggagatg atgaaggaga
361cgttcaagta cttcctccgg ggataccgct acgtgattct ggatatcccc cTGCTGTTTG
421AGACCAAGAA GTTGCTCAAG TACATGAAGC ACACCGTGGT AGTATACTGC GACCGGGACA
481CACAGCTGGC ACGGCTGATG CGGCGGAACA GCCTGAACCG CAAGGACGCA GAGGCCCGCA
541TCAATGCCCA GCTGCCCCTG ACAGACAAGG CCCGCATGGC CCGCCATGTC CTAGACAACT
601CGGGCGAGTG GAGTGTCACC AAACGCCAGG TCATCCTCTT GCACACTGAG CTGGAGCGCT
661CCCTGGAGTA CCTGCCGCTG AGGTTTGGGG TCCTCACAGG GCTCGCTGCC ATTGCCAGCC
721TCCTCTACCT GCTCACCCAC TACCTTCTGC CTTACGCCTA CCCAACTTTC TTGTACAAAG
781TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT
841GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG
901ACGAACGAGT GCAACTTTAC ATCTACATAC GCGTTAAGTC gacaatcaac ctctggatta
961caaaatttgt gaaagatt

Download FASTA (ORF) (Full)