Construct: ORF TRCN0000466056
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000852.1_s317c1
- Derived from:
- ccsbBroadEn_03356
- DNA Barcode:
- GATTGTGGGACCCCCCCAGGACAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CHMP3 (51652)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466056
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51652 | CHMP3 | charged multivesicular body... | NM_016079.4 | 100% | 100% | |
| 2 | human | 100526767 | RNF103-CHMP3 | RNF103-CHMP3 readthrough | NM_001198954.1 | 84.4% | 83.2% | (many diffs) |
| 3 | human | 51652 | CHMP3 | charged multivesicular body... | NM_001193517.2 | 81.9% | 81.9% | 212_213ins120 |
| 4 | human | 51652 | CHMP3 | charged multivesicular body... | NM_001005753.3 | 70.2% | 70.2% | 0_1ins198 |
| 5 | human | 51652 | CHMP3 | charged multivesicular body... | NR_036454.2 | 14.6% | (many diffs) | |
| 6 | mouse | 66700 | Chmp3 | charged multivesicular body... | NM_025783.3 | 89.2% | 95.9% | (many diffs) |
| 7 | mouse | 66700 | Chmp3 | charged multivesicular body... | XM_006506480.3 | 79% | 84.3% | (many diffs) |
| 8 | mouse | 66700 | Chmp3 | charged multivesicular body... | XM_017321713.1 | 79% | 84.3% | (many diffs) |
| 9 | mouse | 66700 | Chmp3 | charged multivesicular body... | XM_006506481.3 | 62.6% | 67.4% | (many diffs) |
| 10 | mouse | 66700 | Chmp3 | charged multivesicular body... | XM_006506482.3 | 62.6% | 67.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 732
- ORF length:
- 666
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg gctgtttgga aagacccagg agaagccgcc caaagaactg gtcaatgagt 121 ggtcattgaa gataagaaag gaaatgagag ttgttgacag gcaaataagg gatatccaaa 181 gagaagaaga aaaagtgaaa cgatctgtga aagatgctgc caagaagggc cagaaggatg 241 tctgcatagt tctggccaag gagatgatca ggtcaaggaa ggctgtgagc aagctgtatg 301 catccaaagc acacatgaac tcagtgctca tggggatgaa gaaccagctc gcggTCTTGC 361 GAGTGGCTGG TTCCCTGCAG AAGAGCACAG AAGTGATGAA GGCCATGCAA AGTCTTGTGA 421 AGATTCCAGA GATTCAGGCC ACCATGAGGG AGTTGTCCAA AGAAATGATG AAGGCTGGGA 481 TCATAGAGGA GATGTTAGAG GACACTTTTG AAAGCATGGA CGATCAGGAA GAAATGGAGG 541 AAGAAGCAGA AATGGAAATT GACAGAATTC TCTTTGAAAT TACAGCAGGG GCCTTGGGCA 601 AAGCACCCAG TAAAGTGACT GATGCCCTTC CAGAGCCAGA ACCTCCAGGA GCGATGGCTG 661 CCTCAGAGGA TGAGGAGGAG GAGGAAGAGG CTCTGGAGGC CATGCAGTCC CGGCTGGCCA 721 CACTCCGCAG CTACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 781 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 841 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAGAT TGTGGGACCC CCCCAGGACA 901 GACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t