Transcript: Mouse NM_027230.5

Mus musculus zinc finger, MYND-type containing 8 (Zmynd8), transcript variant 1, mRNA.

Source:
NCBI, updated 2017-06-26
Taxon:
Mus musculus (mouse)
Gene:
Zmynd8 (228880)
Length:
5423
CDS:
218..3985

Additional Resources:

NCBI RefSeq record:
NM_027230.5
NBCI Gene record:
Zmynd8 (228880)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_027230.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000375473 GCCAAACACTTTAGGTGTAAG pLKO_005 4177 3UTR 100% 10.800 15.120 N Zmynd8 n/a
2 TRCN0000366618 TAACTCGACCCTGGATCTTTC pLKO_005 3679 CDS 100% 10.800 15.120 N Zmynd8 n/a
3 TRCN0000088517 CGTGTGGAACGCATCAAGTAA pLKO.1 2758 CDS 100% 5.625 7.875 N Zmynd8 n/a
4 TRCN0000088513 CGCCACCTTTAGAATTTCCAA pLKO.1 4066 3UTR 100% 3.000 4.200 N Zmynd8 n/a
5 TRCN0000088515 CGGCTTACTCAACAGTAGCAA pLKO.1 433 CDS 100% 3.000 4.200 N Zmynd8 n/a
6 TRCN0000366548 GGAACGCATCAAGTAAGTTTC pLKO_005 2763 CDS 100% 10.800 8.640 N Zmynd8 n/a
7 TRCN0000375472 CCTGCACAACTGCATTATTTA pLKO_005 949 CDS 100% 15.000 10.500 N Zmynd8 n/a
8 TRCN0000088516 GCCTGGGTTCCAGTCAATAAT pLKO.1 1229 CDS 100% 15.000 10.500 N Zmynd8 n/a
9 TRCN0000375531 TACACTGACCTTAACTTATAA pLKO_005 4248 3UTR 100% 15.000 10.500 N Zmynd8 n/a
10 TRCN0000366546 ATCACAAGTTGACGCAAATAG pLKO_005 981 CDS 100% 13.200 9.240 N Zmynd8 n/a
11 TRCN0000088514 GCCCGTTTCTTTGGACAACAT pLKO.1 1202 CDS 100% 4.950 2.970 N Zmynd8 n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_027230.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11753 pDONR223 100% 53.7% 54.3% None (many diffs) n/a
2 ccsbBroad304_11753 pLX_304 0% 53.7% 54.3% V5 (many diffs) n/a
3 TRCN0000476000 TGCCCTTGAGAACTCATACTTATC pLX_317 9.2% 53.7% 54.3% V5 (many diffs) n/a
Download CSV