Transcript: Mouse NM_027756.5

Mus musculus microfibrillar-associated protein 3-like (Mfap3l), transcript variant 1, mRNA.

Source:
NCBI, updated 2017-06-04
Taxon:
Mus musculus (mouse)
Gene:
Mfap3l (71306)
Length:
6372
CDS:
367..1596

Additional Resources:

NCBI RefSeq record:
NM_027756.5
NBCI Gene record:
Mfap3l (71306)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_027756.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000089645 CTAACATCTATGGCACTATAA pLKO.1 749 CDS 100% 13.200 18.480 N Mfap3l n/a
2 TRCN0000089643 CCGTTCTTTCCTAATGGAGTA pLKO.1 2049 3UTR 100% 4.050 5.670 N Mfap3l n/a
3 TRCN0000417571 GACTCGGATGCTTCGTCATTG pLKO_005 1270 CDS 100% 10.800 8.640 N Mfap3l n/a
4 TRCN0000418138 AGACATGGGCGTGTACTATAT pLKO_005 807 CDS 100% 13.200 9.240 N Mfap3l n/a
5 TRCN0000089644 CCTAGAGTTTAAGTGGTATAA pLKO.1 591 CDS 100% 13.200 9.240 N Mfap3l n/a
6 TRCN0000089647 TCTTTCTCAGACAGAGGTAAA pLKO.1 712 CDS 100% 10.800 7.560 N Mfap3l n/a
7 TRCN0000417762 TGGAATTTGCTAGGTACATTG pLKO_005 1049 CDS 100% 10.800 7.560 N Mfap3l n/a
8 TRCN0000089646 CAGAACCATCAGAGGAACATT pLKO.1 1403 CDS 100% 5.625 3.938 N Mfap3l n/a
9 TRCN0000116944 GCCAGAACTGACCATATCATA pLKO.1 517 CDS 100% 5.625 3.938 N MFAP3L n/a
10 TRCN0000166364 CACACACACACACACACACAA pLKO.1 4414 3UTR 100% 4.950 2.475 Y KAAG1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_027756.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11423 pDONR223 100% 22.3% 20.7% None (many diffs) n/a
2 ccsbBroad304_11423 pLX_304 0% 22.3% 20.7% V5 (many diffs) n/a
3 TRCN0000469608 AGTGCGCAACGAGGCCGGCCCCCA pLX_317 100% 22.3% 20.7% V5 (many diffs) n/a
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