Transcript: Human NM_030779.4

Homo sapiens potassium voltage-gated channel subfamily H member 6 (KCNH6), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-08-03
Taxon:
Homo sapiens (human)
Gene:
KCNH6 (81033)
Length:
3866
CDS:
57..3041

Additional Resources:

NCBI RefSeq record:
NM_030779.4
NBCI Gene record:
KCNH6 (81033)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_030779.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000019840 GCCCTACCTAGAACACAAGAT pLKO.1 1337 CDS 100% 4.950 6.930 N KCNH6 n/a
2 TRCN0000019843 CGAGGGCCAAAGTCGGAAGTT pLKO.1 122 CDS 100% 1.650 2.310 N KCNH6 n/a
3 TRCN0000447137 GGTGGGCAAGGTGAGAGTTAA pLKO_005 3075 3UTR 100% 13.200 10.560 N KCNH6 n/a
4 TRCN0000420762 CCACTGAACTCCAAGATAAAG pLKO_005 3035 CDS 100% 13.200 9.240 N KCNH6 n/a
5 TRCN0000019839 CCTGGAAGTACAAGGACTCAT pLKO.1 2909 CDS 100% 4.950 3.465 N KCNH6 n/a
6 TRCN0000019841 CGTGGAGTTCAACTTGGAGAA pLKO.1 620 CDS 100% 4.050 2.835 N KCNH6 n/a
7 TRCN0000019842 CCAAGCTATGGAGACTTGGAT pLKO.1 2751 CDS 100% 0.300 0.210 N KCNH6 n/a
8 TRCN0000442901 GCCTGAGCTACTGCAGGAAAT pLKO_005 2441 CDS 100% 10.800 6.480 N KCNH6 n/a
9 TRCN0000222574 CGCCTGTAATCCCAGCACTTT pLKO.1 3128 3UTR 100% 4.950 2.475 Y ERAP2 n/a
10 TRCN0000078113 GCCTGTAATCCCAGCACTTTA pLKO.1 3129 3UTR 100% 13.200 6.600 Y LIAS n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_030779.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14289 pDONR223 100% 50.3% 50.2% None (many diffs) n/a
2 ccsbBroad304_14289 pLX_304 0% 50.3% 50.2% V5 (not translated due to prior stop codon) (many diffs) n/a
3 TRCN0000468367 GATACGGCATTCACCCGCCGGGGG pLX_317 29% 50.3% 50.2% V5 (not translated due to prior stop codon) (many diffs) n/a
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