Transcript: Human NM_032364.5

Homo sapiens DnaJ heat shock protein family (Hsp40) member C14 (DNAJC14), mRNA.

Source:
NCBI, updated 2019-05-04
Taxon:
Homo sapiens (human)
Gene:
DNAJC14 (85406)
Length:
3330
CDS:
205..2313

Additional Resources:

NCBI RefSeq record:
NM_032364.5
NBCI Gene record:
DNAJC14 (85406)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_032364.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000413422 GAAACGAAGTCAGGCAGATAA pLKO_005 879 CDS 100% 13.200 9.240 N DNAJC14 n/a
2 TRCN0000155054 GCCTTGGGTCAAGCAGAATAT pLKO.1 1407 CDS 100% 13.200 9.240 N DNAJC14 n/a
3 TRCN0000420123 ATTTCTTGAGTCGGATCTTTC pLKO_005 2138 CDS 100% 10.800 7.560 N DNAJC14 n/a
4 TRCN0000151282 CGAAAGGAGTATGAGATGAAA pLKO.1 1702 CDS 100% 5.625 3.938 N DNAJC14 n/a
5 TRCN0000150794 GAGTTGGAAGAGGAATATGAT pLKO.1 700 CDS 100% 5.625 3.938 N DNAJC14 n/a
6 TRCN0000155282 GCCGAGGAACTATGTCAACTT pLKO.1 925 CDS 100% 4.950 3.465 N DNAJC14 n/a
7 TRCN0000152371 CCTATCACATCTCATTTGGTT pLKO.1 2051 CDS 100% 3.000 2.100 N DNAJC14 n/a
8 TRCN0000154777 GCTGAGTGTAATAGGCTGCAT pLKO.1 1882 CDS 100% 2.640 1.848 N DNAJC14 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_032364.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04482 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_04482 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000468353 CTGTACCCTCCTGGTTACTTTCAC pLX_317 16.7% 100% 100% V5 n/a
4 ccsbBroadEn_16052 pDONR223 0% 99.9% 100% None 552A>G n/a
5 ccsbBroad304_16052 pLX_304 0% 99.9% 100% V5 552A>G n/a
6 TRCN0000479570 ACTAATTGCAGCGCTCCTGTCCAG pLX_317 12.8% 99.9% 100% V5 552A>G n/a
7 ccsbBroadEn_12810 pDONR223 100% 21.5% 21.5% None (many diffs) n/a
8 ccsbBroad304_12810 pLX_304 0% 21.5% 21.5% V5 (many diffs) n/a
9 TRCN0000471746 TTGAATTCCGTTTATATTACAGAT pLX_317 39.8% 21.5% 21.5% V5 (many diffs) n/a
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