Transcript: Human NM_033273.2

Homo sapiens zinc finger protein 479 (ZNF479), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-04-26
Taxon:
Homo sapiens (human)
Gene:
ZNF479 (90827)
Length:
2072
CDS:
272..1846

Additional Resources:

NCBI RefSeq record:
NM_033273.2
NBCI Gene record:
ZNF479 (90827)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_033273.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000239328 CTCGAACCTTATGAGACATAG pLKO_005 1366 CDS 100% 10.800 7.560 N ZNF479 n/a
2 TRCN0000257373 GCCTTTAGCTGGTCTGCAAAC pLKO_005 1016 CDS 100% 6.000 3.600 N ZNF479 n/a
3 TRCN0000239327 CTGGTCTGCAAACCTTACTAG pLKO_005 1024 CDS 100% 4.950 2.970 N ZNF479 n/a
4 TRCN0000239326 TCCACCCTCAGGCCTTATAAT pLKO_005 1857 3UTR 100% 15.000 7.500 Y ZNF479 n/a
5 TRCN0000016513 CTCAAACCTTACTAGACATAA pLKO.1 1282 CDS 100% 13.200 6.600 Y ZNF117 n/a
6 TRCN0000096537 CTGGAGAGAAACCCTACAAAT pLKO.1 1734 CDS 100% 13.200 6.600 Y Zfp934 n/a
7 TRCN0000235358 CTGGAGAGAAACCCTACAAAT pLKO_005 1734 CDS 100% 13.200 6.600 Y 2810408B13Rik n/a
8 TRCN0000244342 CTGGAGAGAAACCCTACAAAT pLKO_005 1734 CDS 100% 13.200 6.600 Y EG668616 n/a
9 TRCN0000239325 TAGCTTATCCTCGAACCTTAT pLKO_005 1357 CDS 100% 10.800 5.400 Y ZNF479 n/a
10 TRCN0000019456 ACCTTACTAGACATAAGAGAA pLKO.1 1035 CDS 100% 4.950 2.475 Y ZNF681 n/a
11 TRCN0000016346 CACTGGAGAGAAACCCTACAA pLKO.1 1816 CDS 100% 4.950 2.475 Y ZNF254 n/a
12 TRCN0000016587 CCTGGGTATTGCTGTCTCTAA pLKO.1 433 CDS 100% 4.950 2.475 Y ZNF675 n/a
13 TRCN0000018502 GATGTTAGAGAACTACAGAAA pLKO.1 403 CDS 100% 4.950 2.475 Y ZNF493 n/a
14 TRCN0000235191 ACTGGAGAGAAACCATATAAA pLKO_005 977 CDS 100% 15.000 7.500 Y Gm4767 n/a
15 TRCN0000235210 ACTGGAGAGAAACCATATAAA pLKO_005 977 CDS 100% 15.000 7.500 Y EG666477 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_033273.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_15279 pDONR223 63.5% 69.8% 38.3% None (many diffs) n/a
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