Transcript: Human NM_052893.2

Homo sapiens BTB domain containing 9 (BTBD9), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-08-22
Taxon:
Homo sapiens (human)
Gene:
BTBD9 (114781)
Length:
8730
CDS:
358..2196

Additional Resources:

NCBI RefSeq record:
NM_052893.2
NBCI Gene record:
BTBD9 (114781)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_052893.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000431780 CCAACCGGCAGCACCAATAAA pLKO_005 2177 CDS 100% 15.000 21.000 N BTBD9 n/a
2 TRCN0000420292 GCCCGTGTCTGCAGGTATATT pLKO_005 1498 CDS 100% 15.000 21.000 N BTBD9 n/a
3 TRCN0000241735 AGCCCGTGTCTGCAGGTATAT pLKO_005 1497 CDS 100% 13.200 18.480 N Btbd9 n/a
4 TRCN0000160713 CCGTACATGATTGGGTCAATA pLKO.1 1789 CDS 100% 13.200 18.480 N BTBD9 n/a
5 TRCN0000159435 GATTGTGATGATCGAAGCTAT pLKO.1 1825 CDS 100% 4.950 6.930 N BTBD9 n/a
6 TRCN0000419346 GGGATATGGACCTCAATTATA pLKO_005 1139 CDS 100% 15.000 10.500 N BTBD9 n/a
7 TRCN0000160249 CTGGCTCATAAATATGGATTT pLKO.1 703 CDS 100% 10.800 7.560 N BTBD9 n/a
8 TRCN0000160645 CAGCCTTATTAGATGGTGATA pLKO.1 1235 CDS 100% 4.950 3.465 N BTBD9 n/a
9 TRCN0000160053 CCTGGCTCATAAATATGGATT pLKO.1 702 CDS 100% 4.950 3.465 N BTBD9 n/a
10 TRCN0000194100 GACTCACAACACAGTGAACAA pLKO.1 1530 CDS 100% 4.950 3.465 N Btbd9 n/a
11 TRCN0000158748 GCATTCACAATGCTACTCAAA pLKO.1 616 CDS 100% 4.950 3.465 N BTBD9 n/a
12 TRCN0000161684 GCAGAAGCATTCACAATGCTA pLKO.1 610 CDS 100% 0.300 0.210 N BTBD9 n/a
13 TRCN0000166364 CACACACACACACACACACAA pLKO.1 5577 3UTR 100% 4.950 2.475 Y KAAG1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_052893.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04659 pDONR223 100% 86.1% 85.9% None (many diffs) n/a
2 ccsbBroad304_04659 pLX_304 0% 86.1% 85.9% V5 (many diffs) n/a
3 TRCN0000466788 CTTGAGATGGGCCAGTAACTGTGC pLX_317 21.5% 86.1% 85.9% V5 (many diffs) n/a
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