Transcript: Human NM_053026.4

Homo sapiens myosin light chain kinase (MYLK), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-07-21
Taxon:
Homo sapiens (human)
Gene:
MYLK (4638)
Length:
9906
CDS:
313..5850

Additional Resources:

NCBI RefSeq record:
NM_053026.4
NBCI Gene record:
MYLK (4638)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001147172 TCAGATCATCCAGTTCCCTG pXPR_003 AGG 3523 64% 21 0.2294 MYLK MYLK 77021
2 BRDN0001147034 GAGCCAAGATGTTGTGAGCA pXPR_003 AGG 1186 21% 10 -0.8384 MYLK MYLK 77022
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_053026.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000000936 ACTGTCCTCTATGGCAATGAT pLKO.1 5376 CDS 100% 5.625 7.875 N MYLK n/a
2 TRCN0000199046 CCACCAAAGTTTGCTACCAAG pLKO.1 790 CDS 100% 4.050 5.670 N MYLK n/a
3 TRCN0000000935 GACGGGAACTGCTCTTTAATT pLKO.1 5689 CDS 100% 15.000 10.500 N MYLK n/a
4 TRCN0000195567 CCACAGTATTGCCACAGTTTA pLKO.1 7375 3UTR 100% 13.200 9.240 N MYLK n/a
5 TRCN0000342488 CCACAGTATTGCCACAGTTTA pLKO_005 7375 3UTR 100% 13.200 9.240 N MYLK n/a
6 TRCN0000000937 CTGAAGGACTGCGCTGTTATT pLKO.1 1663 CDS 100% 13.200 9.240 N MYLK n/a
7 TRCN0000342436 CTGAAGGACTGCGCTGTTATT pLKO_005 1663 CDS 100% 13.200 9.240 N MYLK n/a
8 TRCN0000199504 GCACTCAGACTTGCGTCTTTA pLKO.1 6971 3UTR 100% 13.200 9.240 N MYLK n/a
9 TRCN0000195592 CCTACCTTATGGGTTCCATAT pLKO.1 6880 3UTR 100% 10.800 7.560 N MYLK n/a
10 TRCN0000195516 CTCCTGAAGTGATCAACTATG pLKO.1 4991 CDS 100% 10.800 7.560 N MYLK n/a
11 TRCN0000196279 GTGACCAATGTAATCTCAAAG pLKO.1 1135 CDS 100% 10.800 7.560 N MYLK n/a
12 TRCN0000342435 GTGACCAATGTAATCTCAAAG pLKO_005 1135 CDS 100% 10.800 7.560 N MYLK n/a
13 TRCN0000220705 GCTAGATTTGACTGCAAGATT pLKO.1 5581 CDS 100% 5.625 3.938 N Mylk n/a
14 TRCN0000010568 GTTGACAGTAGAAGGAAGTTT pLKO.1 672 CDS 100% 5.625 3.938 N MYLK n/a
15 TRCN0000342434 GTTGACAGTAGAAGGAAGTTT pLKO_005 672 CDS 100% 5.625 3.938 N MYLK n/a
16 TRCN0000010567 GCTAATGAAAGATACCAAGAA pLKO.1 5259 CDS 100% 4.950 3.465 N MYLK n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_053026.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000491341 TTCTTAAGACCGAAAAATAGATTC pLX_317 10.1% 59.7% 59.7% V5 (many diffs) n/a
2 TRCN0000489873 TTTACCTCCTTTTCCTACACCTGC pLX_317 12.6% 59.7% 59.7% V5 (not translated due to prior stop codon) (many diffs) n/a
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