Construct: ORF TRCN0000489873
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020495.1_s317c1
- DNA Barcode:
- TTTACCTCCTTTTCCTACACCTGC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- MYLK (4638)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489873
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4638 | MYLK | myosin light chain kinase | XM_017006469.2 | 89.4% | 89.5% | (many diffs) |
| 2 | human | 4638 | MYLK | myosin light chain kinase | XM_017006471.2 | 64.6% | 64.6% | 0_1ins1170;717T>C;1242T>C |
| 3 | human | 4638 | MYLK | myosin light chain kinase | XM_017006470.2 | 64.5% | 64.5% | (many diffs) |
| 4 | human | 4638 | MYLK | myosin light chain kinase | NM_001321309.2 | 63.4% | 63.4% | (many diffs) |
| 5 | human | 4638 | MYLK | myosin light chain kinase | NM_053026.4 | 59.7% | 59.7% | (many diffs) |
| 6 | human | 4638 | MYLK | myosin light chain kinase | XM_024453535.1 | 59.7% | 59.7% | (many diffs) |
| 7 | human | 4638 | MYLK | myosin light chain kinase | XM_024453534.1 | 59.4% | 59.4% | (many diffs) |
| 8 | human | 4638 | MYLK | myosin light chain kinase | NM_053025.4 | 57.5% | 57.5% | (many diffs) |
| 9 | human | 4638 | MYLK | myosin light chain kinase | XM_024453533.1 | 57.5% | 57.5% | (many diffs) |
| 10 | human | 4638 | MYLK | myosin light chain kinase | XM_024453536.1 | 57.5% | 57.5% | (many diffs) |
| 11 | human | 4638 | MYLK | myosin light chain kinase | XM_024453537.1 | 57.5% | 57.5% | (many diffs) |
| 12 | human | 4638 | MYLK | myosin light chain kinase | XM_024453532.1 | 57.2% | 57.2% | (many diffs) |
| 13 | human | 4638 | MYLK | myosin light chain kinase | XM_011512860.3 | 57.2% | 57.2% | (many diffs) |
| 14 | human | 4638 | MYLK | myosin light chain kinase | NM_053028.4 | 56.9% | 56.9% | (many diffs) |
| 15 | human | 4638 | MYLK | myosin light chain kinase | NM_053027.4 | 54.9% | 54.9% | (many diffs) |
| 16 | human | 4638 | MYLK | myosin light chain kinase | XM_011512861.3 | 53.7% | 53.7% | (many diffs) |
| 17 | human | 4638 | MYLK | myosin light chain kinase | NM_053032.4 | 13.9% | 13.9% | 0_1ins2850 |
| 18 | human | 4638 | MYLK | myosin light chain kinase | XM_017006472.2 | 13.9% | 13.9% | 0_1ins2850 |
| 19 | human | 4638 | MYLK | myosin light chain kinase | NM_053031.4 | 13.8% | 13.8% | 0_1ins2850;84_85insGAA |
| 20 | human | 4638 | MYLK | myosin light chain kinase | XM_017006473.1 | 13.8% | 13.8% | 0_1ins2850;84_85insGAA |
| 21 | mouse | 107589 | Mylk | myosin, light polypeptide k... | XM_006521705.2 | 57.9% | 62.2% | (many diffs) |
| 22 | mouse | 107589 | Mylk | myosin, light polypeptide k... | XM_017316849.1 | 51.2% | 53.2% | (many diffs) |
| 23 | mouse | 107589 | Mylk | myosin, light polypeptide k... | XM_006521702.1 | 50% | 51.9% | (many diffs) |
| 24 | mouse | 107589 | Mylk | myosin, light polypeptide k... | XM_006521703.3 | 50% | 51.9% | (many diffs) |
| 25 | mouse | 107589 | Mylk | myosin, light polypeptide k... | NM_139300.3 | 49.8% | 51.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 3384
- ORF length:
- 3312
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgctacag aacagctctg ccagagccct tccacggggg agggagcctg 121 ccagctgcga ggacctctgt ggtggaggag ttggtgctga tggtggtggt agtgaccgct 181 atgggtccct gaggcctggc tggccagcaa gagggcaggg ttggccagag gaggaagacg 241 gcgaggacgt gcgaggggtg ctgaagaggc gcgtggagac gaggcagcac actgaggagg 301 cgatccgcca gcaggaggtg gagcagctgg acttccgaga cctcctgggg aagaaggtga 361 gtacaaagac cctatcggaa gacgaactga aggagatccc agccgagcag atggatttcc 421 gtgccaacct gcagcggcaa gtgaagccaa agactgtgtc tgaggaagag aggaaggtgc 481 acagccccca gcaggtcgat tttcgctctg tcctggccaa gaaggggact tccaagaccc 541 ccgtgcctga gaaggtgcca ccgccaaaac ctgccacccc ggattttcgc tcagtgctgg 601 gtggcaagaa gaaattacca gcagagaatg gcagcagcag tgccgagacc ctgaatgcca 661 aggcagtgga aagttccaag cccctgagca atgcacagcc ttcagggccc ttgaaacccg 721 tgggcaacgc caagcctgct gagaccctga agccaatggg caacgccaag cctgccgaga 781 ccctgaagcc catgggcaat gccaagcctg atgagaacct gaaatccgct agcaaagaac 841 tcaagaaaga cgttaagaat gatgtgaact gcaagagagg ccatgcaggg accacagata 901 atgaaaagag atcagagagc caggggacag ccccagcctt caagcagaag ctgcaagatg 961 ttcatgtggc agagggcaag aagctgctgc tccagtgcca ggtgtcttct gaccccccag 1021 ccaccatcat ctggacgctg aatggaaaga ccctcaagac caccaagttc atcatcctct 1081 cccaggaagg ctcactctgc tccgtctcca tcgagaaggc actgcctgag gacagaggct 1141 tatacaagtg tgtagccaag aatgacgctg gccaggcgga gtgctcctgc caagtcaccg 1201 tggatgatgc tccagccagt gagaacacca aggccccaga gatgaaatcc cggaggccca 1261 agagctctct tcctcccgtg ctaggaactg agagtgatgc gactgtgaaa aagaaacctg 1321 cccccaagac acctccgaag gcagcaatgc cccctcagat catccagttc cctgaggacc 1381 agaaggtacg cgcaggagag tcagtggagc tgtttggcaa agtgacaggc actcagccca 1441 tcacctgtac ctggatgaag ttccgaaagc agatccagga aagcgagcac atgaaggtgg 1501 agaacagcga gaatggcagc aagctcacca tcctggccgc gcgccaggag cactgcggct 1561 gctacacact gctggtggag aacaagctgg gcagcaggca ggcccaggtc aacctcactg 1621 tcgtggataa gccagacccc ccagctggca caccttgtgc ctctgacatt cggagctcct 1681 cactgaccct gtcctggtat ggctcctcat atgatggggg cagtgctgta cagtcctaca 1741 gcatcgagat ctgggactca gccaacaaga cgtggaagga actagccaca tgccgcagca 1801 cctctttcaa cgtccaggac ctgctgcctg accacgaata taagttccgt gtacgtgcaa 1861 tcaacgtgta tggaaccagt gagccaagcc aggagtctga actcacaacg gtaggagaga 1921 aacctgaaga gccgaaggat gaagtggagg tgtcagacga tgatgagaag gagcccgagg 1981 ttgattaccg gacagtgaca atcaatactg aacaaaaagt atctgacttc tacgacattg 2041 aggagagatt aggatctggg aaatttggac aggtctttcg acttgtagaa aagaaaactc 2101 gaaaagtctg ggcagggaag ttcttcaagg catattcagc aaaagagaaa gagaatatcc 2161 ggcaggagat tagcatcatg aactgcctcc accaccctaa gctggtccag tgtgtggatg 2221 cctttgaaga aaaggccaac atcgtcatgg tcctggagat cgtgtcagga ggggagctgt 2281 ttgagcgcat cattgacgag gactttgagc tgacggagcg tgagtgcatc aagtacatgc 2341 ggcagatctc ggagggagtg gagtacatcc acaagcaggg catcgtgcac ctggacctca 2401 agccggagaa catcatgtgt gtcaacaaga cgggcaccag gatcaagctc atcgactttg 2461 gtctggccag gaggctggag aacgcggggt ctctgaaggt cctctttggc accccagaat 2521 ttgtggctcc tgaagtgatc aactatgagc ccatcggcta cgccacagac atgtggagca 2581 tcggggtcat ctgctacatc ctagtcagtg gcctttcccc cttcatggga gacaacgata 2641 acgaaacctt ggccaacgtt acctcagcca cctgggactt cgacgacgag gcattcgatg 2701 agatctccga cgatgccaag gatttcatca gcaatctgct gaagaaagat atgaaaaacc 2761 gcctggactg cacgcagtgc cttcagcatc catggctaat gaaagatacc aagaacatgg 2821 aggccaagaa actctccaag gaccggatga agaagtacat ggcaagaagg aaatggcaga 2881 aaacgggcaa tgctgtgaga gccattggaa gactgtcctc tatggcaatg atctcagggc 2941 tcagtggcag gaaatcctca acagggtcac caacCAGCCC GCTCAATGCA GAAAAACTAG 3001 AATCTGAAGA AGATGTGTCC CAAGCTTTCC TTGAGGCTGT TGCTGAGGAA AAGCCTCATG 3061 TAAAACCCTA TTTCTCTAAG ACCATTCGCG ATTTAGAAGT TGTGGAGGGA AGTGCTGCTA 3121 GATTTGACTG CAAGATTGAA GGATACCCAG ACCCCGAGGT TGTCTGGTTC AAAGATGACC 3181 AGTCAATCAG GGAGTCCCGC CACTTCCAGA TAGACTACGA TGAGGACGGG AACTGCTCTT 3241 TAATTATTAG TGATGTTTGC GGGGATGACG ATGCCAAGTA CACCTGCAAG GCTGTCAACA 3301 GTCTTGGAGA AGCCACCTGC ACAGCAGAGC TCATTGTGGA AACGATGGAG GAAGGTGAAG 3361 GGGAAGGGGA AGAGGAAGAA GAGTAGGACC CAGCTTTCTT GTACAAAGTG GTTGATATCG 3421 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 3481 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GATTTACCTC 3541 CTTTTCCTAC ACCTGCACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 3601 aagatt