Construct: ORF TRCN0000491341
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019126.2_s317c1
- DNA Barcode:
- TTCTTAAGACCGAAAAATAGATTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MYLK (4638)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491341
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4638 | MYLK | myosin light chain kinase | XM_017006469.2 | 89.5% | 89.6% | (many diffs) |
| 2 | human | 4638 | MYLK | myosin light chain kinase | XM_017006471.2 | 64.5% | 64.5% | (many diffs) |
| 3 | human | 4638 | MYLK | myosin light chain kinase | XM_017006470.2 | 64.4% | 64.4% | (many diffs) |
| 4 | human | 4638 | MYLK | myosin light chain kinase | NM_001321309.2 | 63.4% | 63.4% | (many diffs) |
| 5 | human | 4638 | MYLK | myosin light chain kinase | NM_053026.4 | 59.7% | 59.7% | (many diffs) |
| 6 | human | 4638 | MYLK | myosin light chain kinase | XM_024453535.1 | 59.7% | 59.7% | (many diffs) |
| 7 | human | 4638 | MYLK | myosin light chain kinase | XM_024453534.1 | 59.4% | 59.4% | (many diffs) |
| 8 | human | 4638 | MYLK | myosin light chain kinase | NM_053025.4 | 57.6% | 57.5% | (many diffs) |
| 9 | human | 4638 | MYLK | myosin light chain kinase | XM_024453533.1 | 57.6% | 57.5% | (many diffs) |
| 10 | human | 4638 | MYLK | myosin light chain kinase | XM_024453536.1 | 57.6% | 57.5% | (many diffs) |
| 11 | human | 4638 | MYLK | myosin light chain kinase | XM_024453537.1 | 57.6% | 57.5% | (many diffs) |
| 12 | human | 4638 | MYLK | myosin light chain kinase | XM_024453532.1 | 57.3% | 57.2% | (many diffs) |
| 13 | human | 4638 | MYLK | myosin light chain kinase | XM_011512860.3 | 57.2% | 57.2% | (many diffs) |
| 14 | human | 4638 | MYLK | myosin light chain kinase | NM_053028.4 | 57% | 56.9% | (many diffs) |
| 15 | human | 4638 | MYLK | myosin light chain kinase | NM_053027.4 | 54.9% | 54.9% | (many diffs) |
| 16 | human | 4638 | MYLK | myosin light chain kinase | XM_011512861.3 | 53.7% | 53.7% | (many diffs) |
| 17 | human | 4638 | MYLK | myosin light chain kinase | NM_053032.4 | 13.9% | 13.9% | 0_1ins2853;462_463insG |
| 18 | human | 4638 | MYLK | myosin light chain kinase | XM_017006472.2 | 13.9% | 13.9% | 0_1ins2853;462_463insG |
| 19 | human | 4638 | MYLK | myosin light chain kinase | NM_053031.4 | 13.8% | 13.8% | 0_1ins2853;84_85insGAA;459_460insG |
| 20 | human | 4638 | MYLK | myosin light chain kinase | XM_017006473.1 | 13.8% | 13.8% | 0_1ins2853;84_85insGAA;459_460insG |
| 21 | mouse | 107589 | Mylk | myosin, light polypeptide k... | XM_006521705.2 | 57.9% | 62.1% | (many diffs) |
| 22 | mouse | 107589 | Mylk | myosin, light polypeptide k... | XM_017316849.1 | 51.3% | 53.2% | (many diffs) |
| 23 | mouse | 107589 | Mylk | myosin, light polypeptide k... | XM_006521702.1 | 50.1% | 52% | (many diffs) |
| 24 | mouse | 107589 | Mylk | myosin, light polypeptide k... | XM_006521703.3 | 50.1% | 52% | (many diffs) |
| 25 | mouse | 107589 | Mylk | myosin, light polypeptide k... | NM_139300.3 | 49.9% | 51.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 3390
- ORF length:
- 3318
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgctacag aacagctctg ccagagccct tccacggggg agggagcctg 121 ccagctgcga ggacctctgt ggtggaggag ttggtgctga tggtggtggt agtgaccgct 181 atgggtccct gaggcctggc tggccagcaa gagggcaggg ttggccagag gaggaagacg 241 gcgaggacgt gcgaggggtg ctgaagaggc gcgtggagac gaggcagcac actgaggagg 301 cgatccgcca gcaggaggtg gagcagctgg acttccgaga cctcctgggg aagaaggtga 361 gtacaaagac cctatcggaa gacgaactga aggagatccc agccgagcag atggatttcc 421 gtgccaacct gcagcggcaa gtgaagccaa agactgtgtc tgaggaagag aggaaggtgc 481 acagccccca gcaggtcgat tttcgctctg tcctggccaa gaaggggact tccaagaccc 541 ccgtgcctga gaaggtgcca ccgccaaaac ctgccacccc ggattttcgc tcagtgctgg 601 gtggcaagaa gaaattacca gcagagaatg gcagcagcag tgccgagacc ctgaatgcca 661 aggcagtgga aagttccaag cccctgagca atgcacagcc ttcagggccc ttgaaacccg 721 tgggcaacgc caagcctgct gagaccctga agccaatggg caacgccaag cctgccgaga 781 ccctgaagcc catgggcaat gccaagcctg atgagaacct gaaatccgct agcaaagaag 841 aactcaagaa agacgttaag aatgatgtga actgcaagag aggccatgca gggaccacag 901 ataatgaaaa gagatcagag agccagggga cagccccagc cttcaagcag aagctgcaag 961 atgttcatgt ggcagagggc aagaagctgc tgctccagtg ccaggtgtct tctgaccccc 1021 cagccaccat catctggacg ctgaatggaa agaccctcaa gaccaccaag ttcatcatcc 1081 tctcccagga aggctcactc tgctccgtct ccatcgagaa ggcactgcct gaggacagag 1141 gcttatacaa gtgtgtagcc aagaatgacg ctggccaggc ggagtgctcc tgccaagtca 1201 ccgtggatga tgctccagcc agtgagaaca ccaaggcccc agagatgaaa tcccggaggc 1261 ccaagagctc tcttcctccc gtgctaggaa ctgagagtga tgcgactgtg aaaaagaaac 1321 ctgcccccaa gacacctccg aaggcagcaa tgccccctca gatcatccag ttccctgagg 1381 accagaaggt acgcgcagga gagtcagtgg agctgtttgg caaagtgaca ggcactcagc 1441 ccatcacctg tacctggatg aagttccgaa agcagatcca ggaaagcgag cacatgaagg 1501 tggagaacag cgagaatggc agcaagctca ccatcctggc cgcgcgccag gagcactgcg 1561 gctgctacac actgctggtg gagaacaagc tgggcagcag gcaggcccag gtcaacctca 1621 ctgtcgtgga taagccagac cccccagctg gcacaccttg tgcctctgac attcggagct 1681 cctcactgac cctgtcctgg tatggctcct catatgatgg gggcagtgct gtacagtcct 1741 acagcatcga gatctgggac tcagccaaca agacgtggaa ggaactagcc acatgccgca 1801 gcacctcttt caacgtccag gacctgctgc ctgaccacga atataagttc cgtgtacgtg 1861 caatcaacgt gtatggaacc agtgagccaa gccaggagtc tgaactcaca acggtaggag 1921 agaaacctga agagccgaag gatgaagtgg aggtgtcaga cgatgatgag aaggagcccg 1981 aggttgatta ccggacagtg acaatcaata ctgaacaaaa agtatctgac ttctacgaca 2041 ttgaggagag attaggatct gggaaatttg gacaggtctt tcgacttgta gaaaagaaaa 2101 ctcgaaaagt ctgggcaggg aagttcttca aggcatattc agcaaaagag aaagagaata 2161 tccggcagga gattagcatc atgaactgcc tccaccaccc taagctggtc cagtgtgtgg 2221 atgcctttga agaaaaggcc aacatcgtca tggtcctgga gatcgtgtca ggaggggagc 2281 tgtttgagcg catcattgac gaggactttg agctgacgga gcgtgagtgc atcaagtaca 2341 tgcggcagat ctcggaggga gtggagtaca tccacaagca gggcatcgtg cacctggacc 2401 tcaagccgga gaacatcatg tgtgtcaaca agacgggcac caggatcaag ctcatcgact 2461 ttggtctggc caggaggctg gagaacgcgg ggtctctgaa ggtcctcttt ggcaccccag 2521 aatttgtggc tcctgaagtg atcaactatg agcccatcgg ctacgccaca gacatgtgga 2581 gcatcggggt catctgctac atcctagtca gtggcctttc ccccttcatg ggagacaacg 2641 ataacgaaac cttggccaac gttacctcag ccacctggga cttcgacgac gaggcattcg 2701 atgagatctc cgacgatgcc aaggatttca tcagcaatct gctgaagaaa gatatgaaaa 2761 accgcctgga ctgcacgcag tgccttcagc atccatggct aatgaaagat accaagaaca 2821 tggaggccaa gaaactctcc aaggaccgga tgaagaagta catggcaaga aggaaatggc 2881 agaaaacggg caatgctgtg agagccattg gaagactgtc ctctatggca atgatctcag 2941 ggctcagtgg caggaaatcc tcaacagggt caccaaccag cccgctcaat gcagaaaaac 3001 tagaatctga agaagatgtg tcccaagctt tccttgaggc tgttgctgag gaaaagccTC 3061 ATGTAAAACC CTATTTCTCT AAGACCATTC GCGATTTAGA AGTTGTGGAG GGAAGTGCTG 3121 CTAGATTTGA CTGCAAGATT GAAGGATACC CAGACCCCGA GGTTGTCTGG TTCAAAGATG 3181 ACCAGTCAAT CAGGGAGTCC CGCCACTTCC AGATAGACTA CGATGAGGAC GGGAACTGCT 3241 CTTTAATTAT TAGTGATGTT TGCGGGGATG ACGATGCCAA GTACACCTGC AAGGCTGTCA 3301 ACAGTCTTGG AGAAGCCACC TGCACAGCAG AGCTCATTGT GGAAACGATG GAGGAAGGTG 3361 AAGGGGAAGG GGAAGAGGAA GAAGAGGACC CAGCTTTCTT GTACAAAGTG GTTGATATCG 3421 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 3481 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GATTCTTAAG 3541 ACCGAAAAAT AGATTCACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 3601 aagatt