Transcript: Human NM_130798.2

Homo sapiens synaptosome associated protein 23 (SNAP23), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-08-27
Taxon:
Homo sapiens (human)
Gene:
SNAP23 (8773)
Length:
2491
CDS:
423..899

Additional Resources:

NCBI RefSeq record:
NM_130798.2
NBCI Gene record:
SNAP23 (8773)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_130798.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000218715 GAAACTCATTGACAGCTAAAG pLKO_005 881 CDS 100% 10.800 15.120 N SNAP23 n/a
2 TRCN0000145326 GAACAACTAAACCGCATAGAA pLKO.1 570 CDS 100% 5.625 7.875 N SNAP23 n/a
3 TRCN0000141483 CCTCATATACTTCAGCAGGTT pLKO.1 1162 3UTR 100% 2.640 3.696 N SNAP23 n/a
4 TRCN0000144931 GCTCTTCTAATTGGGAGATAA pLKO.1 991 3UTR 100% 13.200 10.560 N SNAP23 n/a
5 TRCN0000141093 CCTGAACATAGGCAATGAGAT pLKO.1 773 CDS 100% 4.950 3.960 N SNAP23 n/a
6 TRCN0000219097 CAGTATCCTGGGAAATCTAAA pLKO_005 743 CDS 100% 13.200 9.240 N SNAP23 n/a
7 TRCN0000257197 TTGCAGTTATAGCCAATTTAG pLKO_005 1394 3UTR 100% 13.200 9.240 N SNAP23 n/a
8 TRCN0000143926 CCCATTTCTTATTGCACCATT pLKO.1 1873 3UTR 100% 4.950 3.465 N SNAP23 n/a
9 TRCN0000143958 CTGAACATAGGCAATGAGATT pLKO.1 774 CDS 100% 4.950 3.465 N SNAP23 n/a
10 TRCN0000144789 GCAATGAGATTGATGCTCAAA pLKO.1 784 CDS 100% 4.950 3.465 N SNAP23 n/a
11 TRCN0000142094 GCCAGAGCAAAGAAACTCATT pLKO.1 870 CDS 100% 4.950 3.465 N SNAP23 n/a
12 TRCN0000141271 CTCACCAGATTACTGATGAGT pLKO.1 460 CDS 100% 3.000 2.100 N SNAP23 n/a
13 TRCN0000139864 GCCATGAAGAAGGAAGCTGTA pLKO.1 1220 3UTR 100% 4.050 2.430 N SNAP23 n/a
14 TRCN0000164801 CCTCCCAAAGTACTGGGATTA pLKO.1 1760 3UTR 100% 1.080 0.540 Y MAPKAPK5-AS1 n/a
15 TRCN0000165534 GAGACAGGGTTTCACCATGTT pLKO.1 1696 3UTR 100% 4.950 2.475 Y n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_130798.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02013 pDONR223 100% 74.8% 74.4% None 265_266ins159 n/a
2 ccsbBroad304_02013 pLX_304 0% 74.8% 74.4% V5 265_266ins159 n/a
3 TRCN0000470641 TGTAATGATATTTGACCGAATGTC pLX_317 55.8% 74.8% 74.4% V5 (not translated due to frame shift) 265_266ins159 n/a
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