Construct: ORF TRCN0000470641
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011783.1_s317c1
- Derived from:
- ccsbBroadEn_02013
- DNA Barcode:
- TGTAATGATATTTGACCGAATGTC
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SNAP23 (8773)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470641
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8773 | SNAP23 | synaptosome associated prot... | NM_003825.4 | 100% | 100% | |
| 2 | human | 8773 | SNAP23 | synaptosome associated prot... | XM_006720725.3 | 95% | 95% | 265_297del |
| 3 | human | 8773 | SNAP23 | synaptosome associated prot... | XM_017022693.2 | 91.7% | 84.9% | (many diffs) |
| 4 | human | 8773 | SNAP23 | synaptosome associated prot... | XM_017022694.1 | 91.7% | 84.9% | (many diffs) |
| 5 | human | 8773 | SNAP23 | synaptosome associated prot... | XM_017022692.2 | 87.4% | 81% | (many diffs) |
| 6 | human | 8773 | SNAP23 | synaptosome associated prot... | NM_130798.2 | 74.8% | 74.4% | 265_266ins159 |
| 7 | mouse | 20619 | Snap23 | synaptosomal-associated pro... | NM_001177793.1 | 88.3% | 87.2% | (many diffs) |
| 8 | mouse | 20619 | Snap23 | synaptosomal-associated pro... | NM_009222.3 | 88.3% | 87.2% | (many diffs) |
| 9 | mouse | 20619 | Snap23 | synaptosomal-associated pro... | XM_006499057.3 | 88.3% | 87.2% | (many diffs) |
| 10 | mouse | 20619 | Snap23 | synaptosomal-associated pro... | NM_001177792.1 | 84.1% | 82.8% | (many diffs) |
| 11 | mouse | 20619 | Snap23 | synaptosomal-associated pro... | XM_006499054.3 | 84.1% | 82.8% | (many diffs) |
| 12 | mouse | 20619 | Snap23 | synaptosomal-associated pro... | XM_006499055.3 | 84.1% | 82.8% | (many diffs) |
| 13 | mouse | 20619 | Snap23 | synaptosomal-associated pro... | XM_006499056.3 | 84.1% | 82.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 699
- ORF length:
- 633
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga taatctgtca tcagaagaaa ttcaacagag agctcaccag attactgatg 121 agtctctgga aagtacgagg agaatcctgg gtttagccat tgagtctcag gatgcaggaa 181 tcaagaccat cactatgctg gatgaacaaa aggaacaact aaaccgcata gaagaaggct 241 tggaccaaat aaataaggac atgagagaga cagagaagac tttaacagaa ctcaacaaat 301 gctgtggcct ttgtgtctgc ccatgtaata gaacaaagaa ctttGAGTCT GGCAAGGCTT 361 ATAAGACAAC ATGGGGAGAT GGTGGAGAAA ACTCACCTTG CAATGTAGTA TCTAAACAGC 421 CAGGCCCGGT GACAAATGGT CAGCTTCAGC AACCAACAAC GGGAGCAGCC AGTGGTGGAT 481 ACATTAAACG CATAACTAAT GATGCCAGAG AAGATGAAAT GGAAGAGAAC CTGACTCAAG 541 TGGGCAGTAT CCTGGGAAAT CTAAAAGACA TGGCCCTGAA CATAGGCAAT GAGATTGATG 601 CTCAAAATCC ACAAATAAAA CGAATCACAG ACAAGGCTGA CACCAACAGA GATCGTATTG 661 ATATTGCCAA TGCCAGAGCA AAGAAACTCA TTGACAGCTA CCAACTTTCT TGTACAAAGT 721 GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG 781 AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA 841 CGATGTAATG ATATTTGACC GAATGTCACG CGTTAAGTCg acaatcaacc tctggattac 901 aaaatttgtg aaagatt