Transcript: Mouse NM_133488.1

Mus musculus netrin G1 (Ntng1), transcript variant e, mRNA.

Source:
NCBI, updated 2017-05-27
Taxon:
Mus musculus (mouse)
Gene:
Ntng1 (80883)
Length:
5110
CDS:
1096..2478

Additional Resources:

NCBI RefSeq record:
NM_133488.1
NBCI Gene record:
Ntng1 (80883)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_133488.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000094518 CATGCCACTTCGTGTTTGTAT pLKO.1 1999 CDS 100% 5.625 3.938 N Ntng1 n/a
2 TRCN0000094514 CCAGACAATCTGTTAATGTAT pLKO.1 3258 3UTR 100% 5.625 3.938 N Ntng1 n/a
3 TRCN0000094516 GATGTATGTAAGAGCCTGATT pLKO.1 1186 CDS 100% 4.950 3.465 N Ntng1 n/a
4 TRCN0000094515 CCTAGAGAAATCTCTCGACTA pLKO.1 1578 CDS 100% 4.050 2.835 N Ntng1 n/a
5 TRCN0000094517 CCAGCATTTCCAGTATCGGTA pLKO.1 2162 CDS 100% 2.640 1.848 N Ntng1 n/a
6 TRCN0000166364 CACACACACACACACACACAA pLKO.1 1001 5UTR 100% 4.950 2.475 Y KAAG1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_133488.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02693 pDONR223 100% 86.5% 91.3% None (many diffs) n/a
2 ccsbBroad304_02693 pLX_304 0% 86.5% 91.3% V5 (many diffs) n/a
3 TRCN0000492131 AAGAGACCACGCCTCAATATTTAT pLX_317 34.4% 86.5% 91.3% V5 (many diffs) n/a
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