Construct: ORF TRCN0000492131
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004700.1_s317c1
- Derived from:
- ccsbBroadEn_02693
- DNA Barcode:
- AAGAGACCACGCCTCAATATTTAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NTNG1 (22854)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492131
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 22854 | NTNG1 | netrin G1 | NM_001372168.1 | 100% | 100% | |
| 2 | human | 22854 | NTNG1 | netrin G1 | NM_001372171.1 | 100% | 100% | |
| 3 | human | 22854 | NTNG1 | netrin G1 | NM_014917.4 | 100% | 100% | |
| 4 | human | 22854 | NTNG1 | netrin G1 | NM_001330665.2 | 95.2% | 95% | 1088_1153del |
| 5 | human | 22854 | NTNG1 | netrin G1 | NM_001113228.3 | 91.2% | 91% | 1088_1213del |
| 6 | human | 22854 | NTNG1 | netrin G1 | NM_001372169.1 | 91.2% | 91% | 1088_1213del |
| 7 | human | 22854 | NTNG1 | netrin G1 | XM_017000683.2 | 91.2% | 91% | 1088_1213del |
| 8 | human | 22854 | NTNG1 | netrin G1 | NM_001372166.1 | 90.6% | 90.4% | 1088_1222del |
| 9 | human | 22854 | NTNG1 | netrin G1 | NM_001312688.2 | 86.7% | 86.5% | 1088_1288del |
| 10 | human | 22854 | NTNG1 | netrin G1 | XM_011541025.2 | 84.1% | 81.6% | (many diffs) |
| 11 | human | 22854 | NTNG1 | netrin G1 | XM_017000686.2 | 82.8% | 82.6% | 1088_1089delGTinsCG;1092_1092delGins223 |
| 12 | human | 22854 | NTNG1 | netrin G1 | NM_001113226.3 | 81.2% | 81% | 1088_1390del |
| 13 | human | 22854 | NTNG1 | netrin G1 | NM_001372167.1 | 81.2% | 81% | 1088_1390del |
| 14 | human | 22854 | NTNG1 | netrin G1 | NM_001372170.1 | 81.2% | 81% | 1088_1390del |
| 15 | mouse | 80883 | Ntng1 | netrin G1 | NM_001163349.1 | 90.8% | 95.8% | (many diffs) |
| 16 | mouse | 80883 | Ntng1 | netrin G1 | NM_133488.1 | 86.5% | 91.3% | (many diffs) |
| 17 | mouse | 80883 | Ntng1 | netrin G1 | NM_001163350.1 | 82.9% | 87.5% | (many diffs) |
| 18 | mouse | 80883 | Ntng1 | netrin G1 | NM_001163348.1 | 82.4% | 86.7% | (many diffs) |
| 19 | mouse | 80883 | Ntng1 | netrin G1 | XM_011240295.2 | 80.5% | 84.8% | (many diffs) |
| 20 | mouse | 80883 | Ntng1 | netrin G1 | XM_011240294.2 | 78.8% | 83.1% | (many diffs) |
| 21 | mouse | 80883 | Ntng1 | netrin G1 | NM_001163351.1 | 75.9% | 80.1% | (many diffs) |
| 22 | mouse | 80883 | Ntng1 | netrin G1 | XM_011240293.2 | 75.8% | 80% | (many diffs) |
| 23 | mouse | 80883 | Ntng1 | netrin G1 | XM_011240292.2 | 74.2% | 78.3% | (many diffs) |
| 24 | mouse | 80883 | Ntng1 | netrin G1 | NM_030699.2 | 73.8% | 77.7% | (many diffs) |
| 25 | mouse | 80883 | Ntng1 | netrin G1 | XM_006502355.3 | 68.5% | 72.2% | (many diffs) |
| 26 | mouse | 80883 | Ntng1 | netrin G1 | XM_011240291.2 | 68.5% | 72.2% | (many diffs) |
| 27 | mouse | 80883 | Ntng1 | netrin G1 | XR_001783784.1 | 11.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1380
- ORF length:
- 1314
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgta tttgtcaaga ttcctgtcga ttcatgccct ttgggttacg gtgtcctcag 121 tgatgcagcc ctaccctttg gtttggggac attatgattt gtgtaagact cagatttaca 181 cggaagaagg gaaagtttgg gattacatgg cctgccagcc ggaatccacg gacatgacaa 241 aatatctgaa agtgaaactc gatcctccgg atattacctg tggagaccct cctgagacgt 301 tctgtgcaat gggcaatccc tacatgtgca ataatgagtg tgatgcgagt acccctgagc 361 tggcacaccc ccctgagctg atgtttgatt ttgaaggaag acatccctcc acattttggc 421 agtctgccac ttggaaggag tatcccaagc ctctccaggt taacatcact ctgtcttgga 481 gcaaaaccat tgagctaaca gacaacatag ttattacctt tgaatctggg cgtccagacc 541 aaatgatcct ggagaagtct ctcgattatg gacgaacatg gcagccctat cagtattatg 601 ccacagactg cttagatgct tttcacatgg atcctaaatc cgtgaaggat ttatcacagc 661 atacggtctt agaaatcatt tgcacagaag agtactcaac agggtataca acaaatagca 721 aaataatcca ctttgaaatc aaagacaggt tcgcgttttt tgctggacct cgcctacgca 781 atatggcttc cctctacgga cagctggata caaccaagaa actcagagat ttctttacag 841 tcacagacct gaggataagg ctgttaagac cagccgttgg ggaaatattt gtagatgagc 901 tacacttggc acgctacttt tacgcgatct cAGACATAAA GGTGCGAGGA AGGTGCAAGT 961 GTAATCTCCA TGCCACTGTA TGTGTGTATG ACAACAGCAA ATTGACATGC GAATGTGAGC 1021 ACAACACTAC AGGTCCAGAC TGTGGGAAAT GCAAGAAGAA TTATCAGGGC CGACCTTGGA 1081 GTCCAGGCTC CTATCTCCCC ATCCCCAAAG GCACTGCAAA TACCTGTATC CCCAGTATTT 1141 CCAGTATTGG TACGAATGTC TGCGACAACG AGCTCCTGCA CTGCCAGAAC GGAGGGACGT 1201 GCCACAACAA CGTGCGCTGC CTGTGCCCGG CCGCATACAC GGGCATCCTC TGCGAGAAGC 1261 TGCGGTGCGA GGAGGCTGGC AGCTGCGGCT CCGACTCTGG CCAGGGCGCG CCCCCGCACG 1321 GCTCCCCAGC GCTGCTGCTG CTGACCACGC TGCTGGGAAC CGCCAGCCCC CTGGTGTTCT 1381 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1441 GTCTCGATTC TACGTAGTAA TGAACTAGCC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1501 TTTATATATC TTGTGGAAAG GACGAAAGAG ACCACGCCTC AATATTTATA CGCGTTAAGT 1561 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt