Construct: ORF TRCN0000492131

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF004700.1_s317c1
Derived from:
ccsbBroadEn_02693
DNA Barcode:
AAGAGACCACGCCTCAATATTTAT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
NTNG1 (22854)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000492131
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 22854 NTNG1 netrin G1 NM_001372168.1 100% 100%
2 human 22854 NTNG1 netrin G1 NM_001372171.1 100% 100%
3 human 22854 NTNG1 netrin G1 NM_014917.4 100% 100%
4 human 22854 NTNG1 netrin G1 NM_001330665.2 95.2% 95% 1088_1153del
5 human 22854 NTNG1 netrin G1 NM_001113228.3 91.2% 91% 1088_1213del
6 human 22854 NTNG1 netrin G1 NM_001372169.1 91.2% 91% 1088_1213del
7 human 22854 NTNG1 netrin G1 XM_017000683.2 91.2% 91% 1088_1213del
8 human 22854 NTNG1 netrin G1 NM_001372166.1 90.6% 90.4% 1088_1222del
9 human 22854 NTNG1 netrin G1 NM_001312688.2 86.7% 86.5% 1088_1288del
10 human 22854 NTNG1 netrin G1 XM_011541025.2 84.1% 81.6% (many diffs)
11 human 22854 NTNG1 netrin G1 XM_017000686.2 82.8% 82.6% 1088_1089delGTinsCG;1092_1092delGins223
12 human 22854 NTNG1 netrin G1 NM_001113226.3 81.2% 81% 1088_1390del
13 human 22854 NTNG1 netrin G1 NM_001372167.1 81.2% 81% 1088_1390del
14 human 22854 NTNG1 netrin G1 NM_001372170.1 81.2% 81% 1088_1390del
15 mouse 80883 Ntng1 netrin G1 NM_001163349.1 90.8% 95.8% (many diffs)
16 mouse 80883 Ntng1 netrin G1 NM_133488.1 86.5% 91.3% (many diffs)
17 mouse 80883 Ntng1 netrin G1 NM_001163350.1 82.9% 87.5% (many diffs)
18 mouse 80883 Ntng1 netrin G1 NM_001163348.1 82.4% 86.7% (many diffs)
19 mouse 80883 Ntng1 netrin G1 XM_011240295.2 80.5% 84.8% (many diffs)
20 mouse 80883 Ntng1 netrin G1 XM_011240294.2 78.8% 83.1% (many diffs)
21 mouse 80883 Ntng1 netrin G1 NM_001163351.1 75.9% 80.1% (many diffs)
22 mouse 80883 Ntng1 netrin G1 XM_011240293.2 75.8% 80% (many diffs)
23 mouse 80883 Ntng1 netrin G1 XM_011240292.2 74.2% 78.3% (many diffs)
24 mouse 80883 Ntng1 netrin G1 NM_030699.2 73.8% 77.7% (many diffs)
25 mouse 80883 Ntng1 netrin G1 XM_006502355.3 68.5% 72.2% (many diffs)
26 mouse 80883 Ntng1 netrin G1 XM_011240291.2 68.5% 72.2% (many diffs)
27 mouse 80883 Ntng1 netrin G1 XR_001783784.1 11.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1380
ORF length:
1314
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgta tttgtcaaga ttcctgtcga ttcatgccct ttgggttacg gtgtcctcag
121tgatgcagcc ctaccctttg gtttggggac attatgattt gtgtaagact cagatttaca
181cggaagaagg gaaagtttgg gattacatgg cctgccagcc ggaatccacg gacatgacaa
241aatatctgaa agtgaaactc gatcctccgg atattacctg tggagaccct cctgagacgt
301tctgtgcaat gggcaatccc tacatgtgca ataatgagtg tgatgcgagt acccctgagc
361tggcacaccc ccctgagctg atgtttgatt ttgaaggaag acatccctcc acattttggc
421agtctgccac ttggaaggag tatcccaagc ctctccaggt taacatcact ctgtcttgga
481gcaaaaccat tgagctaaca gacaacatag ttattacctt tgaatctggg cgtccagacc
541aaatgatcct ggagaagtct ctcgattatg gacgaacatg gcagccctat cagtattatg
601ccacagactg cttagatgct tttcacatgg atcctaaatc cgtgaaggat ttatcacagc
661atacggtctt agaaatcatt tgcacagaag agtactcaac agggtataca acaaatagca
721aaataatcca ctttgaaatc aaagacaggt tcgcgttttt tgctggacct cgcctacgca
781atatggcttc cctctacgga cagctggata caaccaagaa actcagagat ttctttacag
841tcacagacct gaggataagg ctgttaagac cagccgttgg ggaaatattt gtagatgagc
901tacacttggc acgctacttt tacgcgatct cAGACATAAA GGTGCGAGGA AGGTGCAAGT
961GTAATCTCCA TGCCACTGTA TGTGTGTATG ACAACAGCAA ATTGACATGC GAATGTGAGC
1021ACAACACTAC AGGTCCAGAC TGTGGGAAAT GCAAGAAGAA TTATCAGGGC CGACCTTGGA
1081GTCCAGGCTC CTATCTCCCC ATCCCCAAAG GCACTGCAAA TACCTGTATC CCCAGTATTT
1141CCAGTATTGG TACGAATGTC TGCGACAACG AGCTCCTGCA CTGCCAGAAC GGAGGGACGT
1201GCCACAACAA CGTGCGCTGC CTGTGCCCGG CCGCATACAC GGGCATCCTC TGCGAGAAGC
1261TGCGGTGCGA GGAGGCTGGC AGCTGCGGCT CCGACTCTGG CCAGGGCGCG CCCCCGCACG
1321GCTCCCCAGC GCTGCTGCTG CTGACCACGC TGCTGGGAAC CGCCAGCCCC CTGGTGTTCT
1381ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG
1441GTCTCGATTC TACGTAGTAA TGAACTAGCC CGTAACTTGA AAGTATTTCG ATTTCTTGGC
1501TTTATATATC TTGTGGAAAG GACGAAAGAG ACCACGCCTC AATATTTATA CGCGTTAAGT
1561Cgacaatcaa cctctggatt acaaaatttg tgaaagatt

Download FASTA (ORF) (Full)