Transcript: Mouse NM_133779.2

Mus musculus phosphatidylinositol glycan anchor biosynthesis, class T (Pigt), mRNA.

Source:
NCBI, updated 2017-05-20
Taxon:
Mus musculus (mouse)
Gene:
Pigt (78928)
Length:
2574
CDS:
35..1783

Additional Resources:

NCBI RefSeq record:
NM_133779.2
NBCI Gene record:
Pigt (78928)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_133779.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000077136 CGGTCTCTCAGTGCTATTGAA pLKO.1 643 CDS 100% 5.625 3.938 N Pigt n/a
2 TRCN0000077134 CCCTCTCTTCAACACCTTGTT pLKO.1 1513 CDS 100% 4.950 3.465 N Pigt n/a
3 TRCN0000077135 GCAAGGAGAATAAACCAAGTT pLKO.1 1263 CDS 100% 4.950 3.465 N Pigt n/a
4 TRCN0000077137 GCTGTACAACTCCCATCCTTA pLKO.1 1159 CDS 100% 4.950 2.970 N Pigt n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_133779.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_08326 pDONR223 100% 87% 93.4% None (many diffs) n/a
2 ccsbBroad304_08326 pLX_304 0% 87% 93.4% V5 (many diffs) n/a
3 TRCN0000476868 TGCGATGGTCCAAGTCACGTCTCG pLX_317 14.1% 87% 93.4% V5 (many diffs) n/a
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