Transcript: Mouse NM_133798.3

Mus musculus exonuclease 3'-5' domain containing 2 (Exd2), mRNA.

Source:
NCBI, updated 2017-05-27
Taxon:
Mus musculus (mouse)
Gene:
Exd2 (97827)
Length:
3323
CDS:
233..2185

Additional Resources:

NCBI RefSeq record:
NM_133798.3
NBCI Gene record:
Exd2 (97827)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_133798.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000216762 GTAATGGGCTTAGCCTGAAAT pLKO.1 924 CDS 100% 13.200 10.560 N Exd2 n/a
2 TRCN0000191054 CCCAGGAGATTATTACTTGAT pLKO.1 1540 CDS 100% 4.950 3.960 N Exd2 n/a
3 TRCN0000241906 CGCTTGCCCAGGCTGATATAT pLKO_005 728 CDS 100% 15.000 10.500 N Exd2 n/a
4 TRCN0000241905 ACAAGCTTCTACAGGATTATG pLKO_005 837 CDS 100% 13.200 9.240 N Exd2 n/a
5 TRCN0000241903 CCCAGTTCAGCCTCACTATTT pLKO_005 2608 3UTR 100% 13.200 9.240 N Exd2 n/a
6 TRCN0000241902 CGGCTGGTTGAAGAGGTTAAT pLKO_005 1214 CDS 100% 13.200 9.240 N Exd2 n/a
7 TRCN0000217417 GGCAGATGGTGCTATCTTAAA pLKO.1 787 CDS 100% 13.200 9.240 N Exd2 n/a
8 TRCN0000241904 TGGCAGATGGTGCTATCTTAA pLKO_005 786 CDS 100% 13.200 9.240 N Exd2 n/a
9 TRCN0000191421 CCAACTGTATTACTTGGATTT pLKO.1 2672 3UTR 100% 10.800 7.560 N Exd2 n/a
10 TRCN0000190454 GCAGATTTCAGTGGCTCTCTT pLKO.1 1060 CDS 100% 4.950 3.465 N Exd2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_133798.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03552 pDONR223 100% 66% 66.7% None (many diffs) n/a
2 ccsbBroad304_03552 pLX_304 0% 66% 66.7% V5 (many diffs) n/a
3 TRCN0000473850 AGGAGTAGTTAGACATCTGCCGGA pLX_317 38.6% 66% 66.7% V5 (many diffs) n/a
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