Construct: ORF TRCN0000473850
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017814.1_s317c1
- Derived from:
- ccsbBroadEn_03552
- DNA Barcode:
- AGGAGTAGTTAGACATCTGCCGGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EXD2 (55218)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473850
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55218 | EXD2 | exonuclease 3'-5' domain co... | NM_018199.3 | 100% | 100% | |
| 2 | human | 55218 | EXD2 | exonuclease 3'-5' domain co... | XM_005267817.4 | 100% | 100% | |
| 3 | human | 55218 | EXD2 | exonuclease 3'-5' domain co... | XM_011536908.3 | 100% | 100% | |
| 4 | human | 55218 | EXD2 | exonuclease 3'-5' domain co... | XM_017021421.2 | 100% | 100% | |
| 5 | human | 55218 | EXD2 | exonuclease 3'-5' domain co... | NM_001193360.1 | 79.8% | 79.8% | 1_375del |
| 6 | human | 55218 | EXD2 | exonuclease 3'-5' domain co... | NM_001193361.1 | 79.8% | 79.8% | 1_375del |
| 7 | human | 55218 | EXD2 | exonuclease 3'-5' domain co... | NM_001193362.1 | 79.8% | 79.8% | 1_375del |
| 8 | human | 55218 | EXD2 | exonuclease 3'-5' domain co... | NM_001193363.1 | 79.8% | 79.8% | 1_375del |
| 9 | human | 55218 | EXD2 | exonuclease 3'-5' domain co... | NR_034164.1 | 29% | 1_711del;2200_5122del | |
| 10 | human | 55218 | EXD2 | exonuclease 3'-5' domain co... | NR_034165.1 | 28.1% | 1_870del;2359_5281del | |
| 11 | mouse | 97827 | Exd2 | exonuclease 3'-5' domain co... | XM_011244226.2 | 86.4% | 87.5% | (many diffs) |
| 12 | mouse | 97827 | Exd2 | exonuclease 3'-5' domain co... | XM_006516414.3 | 76.4% | 70.8% | (many diffs) |
| 13 | mouse | 97827 | Exd2 | exonuclease 3'-5' domain co... | NM_133798.3 | 66% | 66.7% | (many diffs) |
| 14 | mouse | 97827 | Exd2 | exonuclease 3'-5' domain co... | XM_006516415.3 | 63.9% | 62.9% | (many diffs) |
| 15 | mouse | 97827 | Exd2 | exonuclease 3'-5' domain co... | XM_011244225.1 | 56.4% | 52.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1554
- ORF length:
- 1488
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ctccccaagt ggcctgtgtg tcttggttcg cctgcccaag ctaatctgtg 121 gaggaaaaac actaccaaga acgttattgg atattttggc agatggcacc attttgaaag 181 ttggagtggg atgctcagaa gatgccagca agcttctgca ggattatggc ctcgttgtta 241 gggggtgcct ggacctccga tacctagcca tgcggcagag aaacaatttg ctctgtaatg 301 ggcttagcct gaagtccctc gctgagactg ttttgaactt tccccttgac aagtcccttc 361 tacttcgttg cagcaactgg gatgctgaga ctctcacaga ggaccaggta atttatgctg 421 ccagggatgc ccagatttca gtggctctct ttcttcatct tcttggatac cctttctcta 481 ggaattcacc tggagaaaaa aacgatgacc acagtagctg gagaaaagtc ttggaaaaat 541 gccagggtgt ggtcgacatc ccatttcgaa gcaaaggaat gagcagattg ggagaagagg 601 ttaatgggga agcaacagaa tctcagcaga agccaagaaa taagaagtct aagatggatg 661 ggatggtgcc aggcaaccac caagggagag accccagaaa acataaaaga aagcctctgg 721 gggtgggcta ttctgccaga aaatcacctc tttatgataa ctgctttctc catgctcctg 781 atggacagcc cctctgcact tgtgatagaa gaaaagctca gtggtacctg gacaaaggca 841 ttggtgagct ggtgagtgaa gagccctttg tggtgaagct acggtttgaa cctgcaggaa 901 ggcccgaatc tcctggagac tattacttga tggttaaaga gaacctgtgt gtagtgtgtg 961 gcaagagaga ctcctacatt cGGAAGAACG TGATTCCACA TGAGTACCGG AAGCACTTCC 1021 CCATCGAGAT GAAGGACCAC AACTCCCACG ATGTGCTGCT GCTCTGCACC TCCTGCCATG 1081 CCATTTCCAA CTACTATGAC AACCATCTGA AGCAGCAGCT GGCCAAGGAG TTCCAGGCCC 1141 CCATCGGCTC TGAGGAGGGC TTGCGCCTGC TGGAAGATCC TGAGCGCCGG CAGGTGCGTT 1201 CTGGGGCCAG GGCCCTGCTC AACGCGGAGA GCCTGCCTAC TCAGCGAAAG GAGGAGCTGC 1261 TGCAAGCACT CAGAGAGTTT TATAACACAG ACGTGGTCAC AGAGGAGATG CTTCAAGAGG 1321 CTGCCAGCCT GGAGACCAGA ATCTCCAATG AAAACTATGT TCCTCACGGG CTGAAGGTGG 1381 TGCAGTGTCA CAGCCAGGGT GGCCTGCGCT CCCTCATGCA GCTGGAGAGC CGCTGGCGTC 1441 AGCACTTCCT GGACTCCATG CAGCCCAAGC ACCTGCCCCA GCAGTGGTCA GTGGACCACA 1501 ACCATCAGAA GCTGCTCCGG AAATTCGGGG AAGATCTTCC CATCCAGCTG TCTTGCCCAA 1561 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1621 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1681 TATCTTGTGG AAAGGACGAA GGAGTAGTTA GACATCTGCC GGAACGCGTT AAGTCgacaa 1741 tcaacctctg gattacaaaa tttgtgaaag att