Transcript: Mouse NM_144937.4

Mus musculus ubiquitin specific peptidase 3 (Usp3), transcript variant 1, mRNA.

Source:
NCBI, updated 2017-06-04
Taxon:
Mus musculus (mouse)
Gene:
Usp3 (235441)
Length:
5594
CDS:
242..1804

Additional Resources:

NCBI RefSeq record:
NM_144937.4
NBCI Gene record:
Usp3 (235441)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_144937.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000030785 GTGTCTTTAGTAGAAGAGTTT pLKO.1 887 CDS 100% 4.950 6.930 N Usp3 n/a
2 TRCN0000030786 CAGAGTTGTATATGTGCCATA pLKO.1 1380 CDS 100% 4.050 5.670 N Usp3 n/a
3 TRCN0000312963 TGCCACAGGCCTTCGAAATTT pLKO_005 712 CDS 100% 15.000 12.000 N Usp3 n/a
4 TRCN0000312964 GTTTCACTGGACAGCATATTT pLKO_005 1480 CDS 100% 15.000 10.500 N Usp3 n/a
5 TRCN0000313032 AGTCGATACATACGTTCAATT pLKO_005 1510 CDS 100% 13.200 9.240 N Usp3 n/a
6 TRCN0000349845 CAACTACACCGGACAACATTT pLKO_005 1826 3UTR 100% 13.200 9.240 N Usp3 n/a
7 TRCN0000007607 CCACTGTGGAAGGTATGTGAA pLKO.1 382 CDS 100% 4.950 3.465 N USP3 n/a
8 TRCN0000030788 CCTTACTCAACCATAAGAGAT pLKO.1 441 CDS 100% 4.950 3.465 N Usp3 n/a
9 TRCN0000030784 GCAAGTAACAAATGCTGCATA pLKO.1 1130 CDS 100% 4.950 3.465 N Usp3 n/a
10 TRCN0000030787 CTGGACATGAAGTGCTACCTA pLKO.1 1544 CDS 100% 3.000 2.100 N Usp3 n/a
11 TRCN0000311936 CTGGACATGAAGTGCTACCTA pLKO_005 1544 CDS 100% 3.000 2.100 N Usp3 n/a
12 TRCN0000106535 GCCTGGTCTACAAAGTGAGTT pLKO.1 4510 3UTR 100% 4.950 2.475 Y Gad2 n/a
13 TRCN0000007605 GCCTCATATGTGGGACAGAAT pLKO.1 1212 CDS 100% 0.000 0.000 N USP3 n/a
14 TRCN0000320688 GCCTCATATGTGGGACAGAAT pLKO_005 1212 CDS 100% 0.000 0.000 N USP3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_144937.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02275 pDONR223 100% 90.7% 96.5% None (many diffs) n/a
2 ccsbBroad304_02275 pLX_304 0% 90.7% 96.5% V5 (many diffs) n/a
3 TRCN0000469117 AATTGGACTGAGCGCTCAGCCACA pLX_317 23.7% 90.7% 96.5% V5 (many diffs) n/a
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