Construct: ORF TRCN0000469117

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF002583.1_s317c1
Derived from:
ccsbBroadEn_02275
DNA Barcode:
AATTGGACTGAGCGCTCAGCCACA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
USP3 (9960)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000469117
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 9960 USP3 ubiquitin specific peptidase 3 NM_006537.4 100% 100%
2 human 9960 USP3 ubiquitin specific peptidase 3 XM_017022763.1 95.5% 94.6% (many diffs)
3 human 9960 USP3 ubiquitin specific peptidase 3 XM_017022764.1 95.5% 94.6% (many diffs)
4 human 9960 USP3 ubiquitin specific peptidase 3 XM_017022765.1 95.5% 94.6% (many diffs)
5 human 9960 USP3 ubiquitin specific peptidase 3 NM_001256702.1 91.5% 91.3% 151_152ins132
6 human 9960 USP3 ubiquitin specific peptidase 3 XM_017022766.1 83.6% 83.6% 0_1ins255
7 human 9960 USP3 ubiquitin specific peptidase 3 XM_017022767.1 83.6% 83.6% 0_1ins255
8 human 9960 USP3 ubiquitin specific peptidase 3 XM_017022768.1 67.5% 67.5% 0_1ins507
9 human 9960 USP3 ubiquitin specific peptidase 3 NR_046342.2 27.6% 1_147del;298_413del;1824_5633del
10 human 9960 USP3 ubiquitin specific peptidase 3 NR_046341.2 27.5% 1_147del;238_390del;1861_5670del
11 mouse 235441 Usp3 ubiquitin specific peptidase 3 NM_144937.4 90.7% 96.5% (many diffs)
12 mouse 235441 Usp3 ubiquitin specific peptidase 3 NM_001302116.1 83.7% 89.2% (many diffs)
13 mouse 235441 Usp3 ubiquitin specific peptidase 3 XM_006511110.3 75.9% 78.9% (many diffs)
14 mouse 235441 Usp3 ubiquitin specific peptidase 3 XM_017313339.1 75.5% 81.1% (many diffs)
15 mouse 235441 Usp3 ubiquitin specific peptidase 3 XM_017313340.1 75.5% 81.1% (many diffs)
16 mouse 235441 Usp3 ubiquitin specific peptidase 3 XM_017313341.1 75.5% 81.1% (many diffs)
17 mouse 235441 Usp3 ubiquitin specific peptidase 3 XM_011242729.2 60.6% 65.3% (many diffs)
18 mouse 235441 Usp3 ubiquitin specific peptidase 3 XM_006511114.3 53.5% 56% (many diffs)
19 mouse 235441 Usp3 ubiquitin specific peptidase 3 NM_001302122.1 46% 50.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1629
ORF length:
1560
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat ggagtgtcca cacctgagct ccagcgtctg cattgctccg gactcagcca
121agttccccaa cggctccccg tcgtcctggt gctgcagcgt gtgccggtcc aacaaaagcc
181cttgggtctg tttgacttgt tcaagtgtcc actgtggaag gtatgtgaat ggccatgcaa
241aaaaacatta tgaagatgca caagtacctt taaccaacca taagaaatca gaaaagcaag
301ataaagttca gcacacagta tgtatggatt gcagtagcta cagtacatac tgttatcgct
361gtgatgattt tgtggttaat gacaccaagc tgggactggt acagaaagtc agagaacact
421tacagaactt ggaaaactca gctttcacag ctgacaggca taagaaaaga aaacttttgg
481aaaactcaac actaaacagc aagttattaa aagtaaatgg aagcaccact gccatttgtg
541ccacaggcct tcggaatttg gggaacacat gtttcatgaa tgccatcctt cagtcactca
601gtaacattga gcagttttgc tgttatttca aagaactgcc cgccgtggag ttaaggaatg
661ggaaaacagc aggaaggcgg acataccaca ccaggagcca aggggataac aatgtgtctt
721tggtagaaga gtttagaaag acactctgtg ctttatggca aggcagccag actgcattta
781gcccagagtc cttattttat gttgtttgga agattatgcc aaactttagg ggctatcaac
841agcaggacgc ccatgaattc atgcgctacc ttttggacca cctacacttg gaacttcagg
901gcggtttcaa cggtgtttcc cgctcagcaa ttctgcagga gaattctact ctgtctgcaa
961gtaacaagtg ttgcataaat ggagcatcta ctgttgtcac ggctatattc ggaggcattc
1021tccaaaatga ggttaactgc ctcatatgtg ggacagaatc tagaaagttt gatccattcc
1081tagacctttc attagatatt ccaagtcagt tcagaagtaa gcgctctaag aatcaagaaa
1141atggaccagt ttgttcgtta cgagattgtc ttcgcagttt taccgactta gaagaacttg
1201atgagacaga gttatatatg tgccataaat gcaaaaagaa acaaaagtcc acaaaaaagt
1261tttGGATTCA AAAACTACCC AAGGTGCTAT GCTTACATTT GAAAAGATTT CATTGGACAG
1321CATATTTAAG AAATAAAGTT GATACATACG TAGAATTTCC ACTGAGAGGC CTAGACATGA
1381AATGCTACTT ACTAGAGCCT GAGAACAGTG GCCCGGAGAG CTGCCTGTAT GACCTCGCCG
1441CTGTGGTGGT GCACCATGGT TCCGGGGTTG GTTCTGGACA TTACACAGCA TACGCAACTC
1501ACGAAGGCCG CTGGTTCCAC TTCAATGACA GTACTGTAAC ACTGACTGAC GAGGAGACTG
1561TGGTGAAGGC GAAGGCCTAC ATCCTTTTCT ACGTGGAACA CCAGGCCAAA GCTGGATCGG
1621ATAAACTTTT GCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC
1681CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA
1741TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAAATTGG ACTGAGCGCT CAGCCACAAC
1801GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt

Download FASTA (ORF) (Full)