Transcript: Human NM_145793.5

Homo sapiens GDNF family receptor alpha 1 (GFRA1), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-07-31
Taxon:
Homo sapiens (human)
Gene:
GFRA1 (2674)
Length:
9052
CDS:
197..1579

Additional Resources:

NCBI RefSeq record:
NM_145793.5
NBCI Gene record:
GFRA1 (2674)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_145793.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000060629 CCAACTACATAGACTCCAGTA pLKO.1 1077 CDS 100% 4.050 5.670 N GFRA1 n/a
2 TRCN0000060632 CGACGACATTTGCAAGAAGTA pLKO.1 670 CDS 100% 4.950 3.960 N GFRA1 n/a
3 TRCN0000060631 GCAGGGTCTGAGAATGAAATT pLKO.1 1322 CDS 100% 13.200 9.240 N GFRA1 n/a
4 TRCN0000060628 CCTCTGTATTTCCAATGGTAA pLKO.1 1417 CDS 100% 4.950 3.465 N GFRA1 n/a
5 TRCN0000060630 GCGCATTTACTGGAGCATGTA pLKO.1 496 CDS 100% 4.950 3.465 N GFRA1 n/a
6 TRCN0000166364 CACACACACACACACACACAA pLKO.1 7967 3UTR 100% 4.950 2.475 Y KAAG1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_145793.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000468740 CACCCGGGCCTTACATTGGCTGAT pLX_317 16.2% 99.9% 100% V5 537C>T n/a
2 ccsbBroadEn_06270 pDONR223 100% 99.8% 99.7% None 537C>T;1364C>N n/a
3 ccsbBroad304_06270 pLX_304 0% 99.8% 99.7% V5 537C>T;1364C>N n/a
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