Construct: ORF TRCN0000468740
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013780.1_s317c1
- Derived from:
- ccsbBroadEn_06270
- DNA Barcode:
- CACCCGGGCCTTACATTGGCTGAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GFRA1 (2674)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468740
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2674 | GFRA1 | GDNF family receptor alpha 1 | NM_001145453.2 | 99.9% | 100% | 537C>T |
| 2 | human | 2674 | GFRA1 | GDNF family receptor alpha 1 | NM_001348096.1 | 99.9% | 100% | 537C>T |
| 3 | human | 2674 | GFRA1 | GDNF family receptor alpha 1 | NM_145793.5 | 99.9% | 100% | 537C>T |
| 4 | human | 2674 | GFRA1 | GDNF family receptor alpha 1 | NM_001348098.2 | 98.8% | 98.9% | 417_431del;552C>T |
| 5 | human | 2674 | GFRA1 | GDNF family receptor alpha 1 | NM_005264.6 | 98.8% | 98.9% | 417_431del;552C>T |
| 6 | human | 2674 | GFRA1 | GDNF family receptor alpha 1 | XM_011539634.2 | 98.8% | 98.9% | 417_431del;552C>T |
| 7 | human | 2674 | GFRA1 | GDNF family receptor alpha 1 | NM_001348099.1 | 73.1% | 70% | (many diffs) |
| 8 | mouse | 14585 | Gfra1 | glial cell line derived neu... | NM_001285457.2 | 88.4% | 92.8% | (many diffs) |
| 9 | mouse | 14585 | Gfra1 | glial cell line derived neu... | NM_010279.3 | 87.5% | 91.8% | (many diffs) |
| 10 | mouse | 14585 | Gfra1 | glial cell line derived neu... | XM_006526681.1 | 87.5% | 91.8% | (many diffs) |
| 11 | mouse | 14585 | Gfra1 | glial cell line derived neu... | XM_006526682.1 | 87.5% | 91.8% | (many diffs) |
| 12 | mouse | 14585 | Gfra1 | glial cell line derived neu... | XM_006526683.3 | 87.5% | 91.8% | (many diffs) |
| 13 | mouse | 14585 | Gfra1 | glial cell line derived neu... | XM_006526684.1 | 87.5% | 91.8% | (many diffs) |
| 14 | mouse | 14585 | Gfra1 | glial cell line derived neu... | NR_104342.1 | 25.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1446
- ORF length:
- 1380
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt cctggcgacc ctgtacttcg cgctgccgct cttggacttg ctcctgtcgg 121 ccgaagtgag cggcggagac cgcctggatt gcgtgaaagc cagtgatcag tgcctgaagg 181 agcagagctg cagcaccaag taccgcacgc taaggcagtg cgtggcgggc aaggagacca 241 acttcagcct ggcatccggc ctggaggcca aggatgagtg ccgcagcgcc atggaggccc 301 tgaagcagaa gtcgctctac aactgccgct gcaagcgggg tatgaagaag gagaagaact 361 gcctgcgcat ttactggagc atgtaccaga gcctgcaggg aaatgatctg ctggaggatt 421 ccccatatga accagttaac agcagattgt cagatatatt ccgggtggtc ccattcatat 481 cagtggagca cattcccaaa gggaacaact gcctggatgc agcgaaggcc tgcaacctcg 541 acgacatttg caagaagtac aggtcggcgt acatcacccc gtgcaccacc agcgtgtcca 601 atgatgtctg caaccgccgc aagtgccaca aggccctccg gcagttcttt gacaaggtcc 661 cggccaagca cagctacgga atgctcttct gctcctgccg ggacatcgcc tgcacagagc 721 ggaggcgaca gaccatcgtg cctgtgtgct cctatgaaga gagggagaag cccaactgtt 781 tgaatttgca ggactcctgc aagacgaatt acatctgcag atctcgcctt gcggattttt 841 ttaccaactg ccagccagag tcaaggtctg tcagcagctg tctaaaggaa aactacgctg 901 actgcctcct cgcctactcg gggcttattg gcacagtcat gacccccaac tacatagact 961 ccagtagcct cagtgtggcc ccatggtgtg actgcagcaa cagtgggaac gacctagaag 1021 agtgcttgaa atttttgaat ttcttcaagg acaatacatg tcttaaaaat gcaattcaag 1081 cctttggcaa tggctccgat gtgaccgtgt ggcagccagc cttcccagta cagaccacca 1141 ctgccactac caccactgcc ctccgggtta agaacaagcc cctggggcca gcagggtctg 1201 agaatgaaat tcccactcat gttttgccaC CGTGTGCAAA TTTACAGGCA CAGAAGCTGA 1261 AATCCAATGT GTCGGGCAAT ACACACCTCT GTATTTCCAA TGGTAATTAT GAAAAAGAAG 1321 GTCTCGGTGC TTCCAGCCAC ATAACCACAA AATCAATGGC TGCTCCTCCA AGCTGTGGTC 1381 TGAGCCCACT GCTGGTCCTG GTGGTAACCG CTCTGTCCAC CCTATTATCT TTAACAGAAA 1441 CATCATACCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1501 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1561 CTTGGCTTTA TATATCTTGT GGAAAGGACG ACACCCGGGC CTTACATTGG CTGATACGCG 1621 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt