Transcript: Mouse NM_153389.3

Mus musculus ATPase, class V, type 10D (Atp10d), transcript variant 1, coding, mRNA.

Source:
NCBI, updated 2017-05-27
Taxon:
Mus musculus (mouse)
Gene:
Atp10d (231287)
Length:
6021
CDS:
100..4509

Additional Resources:

NCBI RefSeq record:
NM_153389.3
NBCI Gene record:
Atp10d (231287)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_153389.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000452027 GAAAGCACCGCGTCGTTATTC pLKO_005 401 CDS 100% 13.200 18.480 N Atp10d n/a
2 TRCN0000101521 GCGCACTCTATGTGTAGCAAA pLKO.1 2769 CDS 100% 4.950 6.930 N Atp10d n/a
3 TRCN0000448969 TGAAGCTTGGGCAGATCTATT pLKO_005 1442 CDS 100% 13.200 10.560 N Atp10d n/a
4 TRCN0000101524 GCAGGGTTTGACTACTGTCAT pLKO.1 1624 CDS 100% 4.950 3.960 N Atp10d n/a
5 TRCN0000101520 GCAGAGTTTAGGAACTGGAAT pLKO.1 4711 3UTR 100% 4.950 3.465 N Atp10d n/a
6 TRCN0000101523 GCTTCAAAGATGAGTACGAAA pLKO.1 434 CDS 100% 4.950 3.465 N Atp10d n/a
7 TRCN0000101522 CCGAGGTTTCTTTACCGAGTT pLKO.1 4186 CDS 100% 4.050 2.835 N Atp10d n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_153389.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_12336 pDONR223 100% 30.6% 31.4% None (many diffs) n/a
2 ccsbBroad304_12336 pLX_304 0% 30.6% 31.4% V5 (many diffs) n/a
3 TRCN0000468955 TCAGCGCCGAGCTATCAGGTTTAT pLX_317 23.8% 30.6% 31.4% V5 (many diffs) n/a
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