Construct: ORF TRCN0000481434

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF002504.1_s317c1
Derived from:
ccsbBroadEn_08258
DNA Barcode:
CCCTCCTTGTTAAAGCGGCATCAG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PHF20 (51230)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000481434
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 51230 PHF20 PHD finger protein 20 NM_016436.5 99.9% 99.9% 1813G>A
2 human 51230 PHF20 PHD finger protein 20 XM_017027868.2 94.5% 94.4% 255_256ins165;1648G>A
3 human 51230 PHF20 PHD finger protein 20 XM_017027869.2 88.5% 85.5% 0_1ins268;71_72ins80;1465G>A
4 human 51230 PHF20 PHD finger protein 20 XM_017027870.2 88.5% 85.5% 0_1ins268;71_72ins80;1465G>A
5 human 51230 PHF20 PHD finger protein 20 XM_024451889.1 88.5% 85.5% 0_1ins268;71_72ins80;1465G>A
6 human 51230 PHF20 PHD finger protein 20 XM_005260421.3 60.1% 60% 0_1ins1209;604G>A
7 human 51230 PHF20 PHD finger protein 20 XM_011528843.2 60.1% 60% 0_1ins1209;604G>A
8 human 51230 PHF20 PHD finger protein 20 XM_011528844.2 60.1% 60% 0_1ins1209;604G>A
9 human 51230 PHF20 PHD finger protein 20 XM_017027871.2 60.1% 60% 0_1ins1209;604G>A
10 human 51230 PHF20 PHD finger protein 20 XM_024451890.1 53.3% 53.2% 0_1ins1416;397G>A
11 mouse 228829 Phf20 PHD finger protein 20 NM_172674.2 87.1% 88.5% (many diffs)
12 mouse 228829 Phf20 PHD finger protein 20 XM_017317827.1 82% 83.3% (many diffs)
13 mouse 228829 Phf20 PHD finger protein 20 XM_006499338.3 73.4% 74.3% (many diffs)
14 mouse 228829 Phf20 PHD finger protein 20 XM_006499339.1 52.1% 53.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
3105
ORF length:
3036
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat gacaaagcat ccacctaaca gacgaggaat cagctttgaa gtgggagccc
121agttggaagc ccgggaccgt ttaaaaaact ggtatccagc tcacatagaa gacattgact
181acgaggaagg aaaagtactc atccatttca agcgttggaa ccatcgttat gatgagtggt
241tctgctggga cagtccttat ttacgccctt tagagaaaat acagctgagg aaagagggct
301tgcatgaaga ggatggatct tctgaatttc aaataaatga gcaggtcctt gcttgctggt
361ctgattgtcg tttttacccg gccaaagtca ctgctgttaa caaggatggt acttacactg
421tgaaatttta tgatggagta gttcagactg tcaaacatat tcatgtcaaa gctttttcca
481aagatcagaa tattgtgggt aatgctaggc ctaaagaaac agatcacaaa agtctttcat
541catctcctga taaacgagag aagtttaaag aacagagaaa agcaacagtg aatgtgaaga
601aagacaaaga agataaaccc ttaaagacag aaaagcgacc caagcagcct gataaagaag
661gaaagttaat ctgttctgaa aaggggaaag tgtcagagaa aagtcttccc aagaacgaga
721aggaagacaa ggaaaacatt tccgaaaatg acagagagta ttctggagat gcccaagtgg
781ataagaaacc tgaaaatgac attgtgaaga gtccacaaga aaacttgagg gaacccaaaa
841gaaaacgagg cagaccccct tccatagctc ctactgctgt ggattcaaac tctcaaactt
901tgcaaccaat aacattggaa ctgagaagaa ggaaaatatc aaaaggatgt gaagtcccat
961taaaacgtcc tcggcttgac aaaaattcat cccaggaaaa gtcaaaaaac tactcggaaa
1021acactgacaa agacttatcg aggagacgtt cctccaggct gtccactaat gggacccatg
1081agatcctaga tcctgacttg gttgtatcag atttggttga tacggatcct ttgcaagaca
1141cgttgtctag taccaaggaa tctgaagaag gtcagttgaa gtctgctttg gaagctggcc
1201aggtctcatc tgcactgact tgccactcct ttggggatgg atccggggct gcaggcttgg
1261agttgaactg cccatcaatg ggagaaaaca cgatgaaaac agaaccgact tctccccttg
1321tggaattaca agagatttcg actgtggaag taacaaatac ttttaagaaa acagatgatt
1381ttgggtcatc taatgcacca gctgtcgacc tagaccataa gtttagatgc aaagttgtgg
1441actgtttaaa atttttccgc aaagccaaac tgttgcacta tcacatgaag tatttccatg
1501gaatggagaa gtcactggag ccagaagaga gcccgggaaa gaggcatgtc caaaccaggg
1561gcccttcagc ttcagacaag cccagccagg agaccctgac caggaagcgg gtctctgcca
1621gttccccaac tacaaaagac aaggaaaaga ataaagagaa gaaattcaag gagtttgtga
1681gagtgaagcc aaagaagaaa aagaaaaaga aaaagaaaac caaacctgaa tgcccctgca
1741gtgaggagat cagtgacacc tcccaggaac cttctccacc caaggcattt gctgttacca
1801ggtgtgggtc ctcacacaag ccaggggtcc atatgagccc gcagcttcat ggcccagaat
1861ctggacacca caaagggaaa atgaaagcat tggaggagga taatttgagt gagtcctctt
1921ctgagagctt tctctggagt gatgatgagt atggccaaga tgtggatgtg accaccaacc
1981cagatgagga acttgatggg gatgaccgct atgacttcga ggtggtccgc tgcatctgtg
2041aggtccagga ggaaaatgac ttcatgattc agtgtgaaga gtgccagtgc tggcagcatg
2101gggtctgcat gggattactg gaagaaaatg tgcccgagaa atacacctgt tatgtttgcc
2161aagaccctcc aggtcagagg cctggcttca agtactggta tgacaaggag tggctgagca
2221ggggacatat gcatggcctg gcatttctag aagagaacta ctcccatcag aatgccaaga
2281agatcgtggc cacccaccag cttcttggtg atgtgcagag agtgattgag gttctgcatg
2341gcctgcagct caagatgagc atcttgcaaa gccgggagca tcctgatctg ccgctgtggt
2401gccagccttg gaaacagcac tcaggggagg ggagatctca tttcagaaac atccctgtca
2461ctgacaccag gagcaaggag gaagctccaa gctatagaac tttgaacggg gcagtggaga
2521agcccaggcc cctggccctg cccctgccgc gttctgtgga ggaatcctat atcaccagtg
2581agcattgcta ccagaagccc cgcgcctatt accctgccgt ggagcagaag ctggtggtgg
2641agacgagggg ctctgccctc gacgatgcgg tcaacccccT CCATGAGAAC GGCGATGATT
2701CCCTTTCCCC GCGCCTGGGC TGGCCTCTAG ACCAAGACAG GAGCAAGGGG GACAGTGACC
2761CCAAACCCGG CTCCCCAAAG GTGAAGGAAT ATGTCTCCAA AAAGGCCCTA CCAGAAGAAG
2821CCCCTGCTCG GAAGCTGCTG GACAGAGGTG GAGAGGGGCT GCTGAGCTCC CAGCACCAGT
2881GGCAGTTTAA CCTGCTGACC CATGTGGAAT CTCTTCAGGA TGAAGTTACG CACAGGATGG
2941ACTCCATTGA GAAGGAGTTG GATGTGTTGG AGAGCTGGCT GGACTACACT GGGGAACTGG
3001AGCCCCCTGA GCCGCTGGCC AGGCTTCCGC AGCTCAAGCA TTGTATCAAG CAGCTGCTGA
3061TGGACCTGGG CAAGGTGCAG CAGATCGCCC TCTGCTGCTC AACATTGCCA ACTTTCTTGT
3121ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT
3181AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG
3241GAAAGGACGA CCCTCCTTGT TAAAGCGGCA TCAGACGCGT TAAGTCgaca atcaacctct
3301ggattacaaa atttgtgaaa gatt

Download FASTA (ORF) (Full)