Transcript: Human NM_178156.2

Homo sapiens fucosyltransferase 8 (FUT8), transcript variant 3, mRNA.

Source:
NCBI, updated 2019-07-06
Taxon:
Homo sapiens (human)
Gene:
FUT8 (2530)
Length:
5081
CDS:
1630..3357

Additional Resources:

NCBI RefSeq record:
NM_178156.2
NBCI Gene record:
FUT8 (2530)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_178156.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000035950 CCACAATCAAATTGCCATTTA pLKO.1 3141 CDS 100% 13.200 18.480 N FUT8 n/a
2 TRCN0000035951 CGTGGAGTGATCCTGGATATA pLKO.1 2971 CDS 100% 13.200 18.480 N FUT8 n/a
3 TRCN0000035952 GTCTATAATGACGGATCTATA pLKO.1 2112 CDS 100% 13.200 18.480 N FUT8 n/a
4 TRCN0000229958 GTCTATAATGACGGATCTATA pLKO_005 2112 CDS 100% 13.200 18.480 N FUT8 n/a
5 TRCN0000229961 TACCCATGCACAGTACAATAA pLKO_005 3561 3UTR 100% 0.000 0.000 N FUT8 n/a
6 TRCN0000218547 CATTGGGATGGCTATTCTAAA pLKO_005 3232 CDS 100% 13.200 10.560 N FUT8 n/a
7 TRCN0000035949 CCTCTTTAACAAGGGCTGCAA pLKO.1 3532 3UTR 100% 2.640 2.112 N FUT8 n/a
8 TRCN0000035953 CCACAGATGACCCTTCTTTAT pLKO.1 2849 CDS 100% 13.200 9.240 N FUT8 n/a
9 TRCN0000229960 CTTTAGATGACATCTACTATT pLKO_005 3104 CDS 100% 13.200 9.240 N FUT8 n/a
10 TRCN0000229959 GAACTGGTTCAGCGGAGAATA pLKO_005 2194 CDS 100% 13.200 9.240 N FUT8 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_178156.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14648 pDONR223 76.1% 99.5% 31.6% None (many diffs) n/a
2 ccsbBroad304_14648 pLX_304 0% 99.5% 31.6% V5 (not translated due to prior stop codon) (many diffs) n/a
3 TRCN0000469624 AGGCAGGCCTAAAATAGTCCCAAC pLX_317 21.8% 99.5% 31.6% V5 (not translated due to prior stop codon) (many diffs) n/a
4 ccsbBroadEn_10831 pDONR223 100% 53.4% 47.2% None (many diffs) n/a
5 ccsbBroad304_10831 pLX_304 0% 53.4% 47.2% V5 (many diffs) n/a
6 TRCN0000475794 GCAGACAGAAAGGTCGGGCGAGAA pLX_317 35.3% 53.4% 47.2% V5 (many diffs) n/a
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