Transcript: Human NM_197967.2

Homo sapiens BH3 interacting domain death agonist (BID), transcript variant 3, mRNA.

Source:
NCBI, updated 2019-09-21
Taxon:
Homo sapiens (human)
Gene:
BID (637)
Length:
2147
CDS:
392..691

Additional Resources:

NCBI RefSeq record:
NM_197967.2
NBCI Gene record:
BID (637)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_197967.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000062709 CTTTCACACAACAGTGAATTT pLKO.1 613 CDS 100% 1.320 1.056 N BID n/a
2 TRCN0000327897 CTTTCACACAACAGTGAATTT pLKO_005 613 CDS 100% 1.320 1.056 N BID n/a
3 TRCN0000312746 GAAGACATCATCCGGAATATT pLKO_005 341 5UTR 100% 15.000 10.500 N BID n/a
4 TRCN0000312688 ATGTCCATTTACACGTATTTG pLKO_005 893 3UTR 100% 13.200 9.240 N BID n/a
5 TRCN0000062711 GTGAGGAGCTTAGCCAGAAAT pLKO.1 659 CDS 100% 13.200 9.240 N BID n/a
6 TRCN0000327898 GTGAGGAGCTTAGCCAGAAAT pLKO_005 659 CDS 100% 13.200 9.240 N BID n/a
7 TRCN0000062712 CAGGGATGAGTGCATCACAAA pLKO.1 136 5UTR 100% 4.950 3.465 N BID n/a
8 TRCN0000327896 CAGGGATGAGTGCATCACAAA pLKO_005 136 5UTR 100% 4.950 3.465 N BID n/a
9 TRCN0000062708 CCTCCAAAGCTGTTCTGACAA pLKO.1 175 5UTR 100% 4.950 3.465 N BID n/a
10 TRCN0000062710 TGGGAAGAATAGAGGCAGATT pLKO.1 309 5UTR 100% 4.950 3.465 N BID n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_197967.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00161 pDONR223 100% 50.7% 50.7% None 0_1ins288 n/a
2 ccsbBroad304_00161 pLX_304 0% 50.7% 50.7% V5 0_1ins288 n/a
3 ccsbBroadEn_05891 pDONR223 100% 50.5% 50.7% None 0_1ins288;240T>C n/a
4 ccsbBroadEn_15368 pDONR223 0% 50.4% 50.2% None 0_1ins288;198C>A;240T>C n/a
5 ccsbBroad304_15368 pLX_304 0% 50.4% 50.2% V5 0_1ins288;198C>A;240T>C n/a
6 TRCN0000472971 ACAAAGGCACGTTGATGTCAATTG pLX_317 86.4% 50.4% 50.2% V5 0_1ins288;198C>A;240T>C n/a
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