Construct: ORF TRCN0000472971
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002872.1_s317c1
- Derived from:
- ccsbBroadEn_15368
- DNA Barcode:
- ACAAAGGCACGTTGATGTCAATTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BID (637)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472971
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 637 | BID | BH3 interacting domain deat... | NM_001196.3 | 99.6% | 99.4% | 486C>A;528T>C |
| 2 | human | 637 | BID | BH3 interacting domain deat... | NM_001244567.1 | 99.6% | 99.4% | 486C>A;528T>C |
| 3 | human | 637 | BID | BH3 interacting domain deat... | NM_197966.2 | 80.6% | 80.4% | 1_138del;624C>A;666T>C |
| 4 | human | 637 | BID | BH3 interacting domain deat... | NM_001244569.1 | 50.4% | 50.2% | 0_1ins288;198C>A;240T>C |
| 5 | human | 637 | BID | BH3 interacting domain deat... | NM_001244570.1 | 50.4% | 50.2% | 0_1ins288;198C>A;240T>C |
| 6 | human | 637 | BID | BH3 interacting domain deat... | NM_001244572.1 | 50.4% | 50.2% | 0_1ins288;198C>A;240T>C |
| 7 | human | 637 | BID | BH3 interacting domain deat... | NM_197967.2 | 50.4% | 50.2% | 0_1ins288;198C>A;240T>C |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 651
- ORF length:
- 585
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ctgtgaggtc aacaacggtt ccagcctcag ggatgagtgc atcacaaacc 121 tactggtgtt tggcttccTC CAAAGCTGTT CTGACAACAG CTTCCGCAGA GAGCTGGACG 181 CACTGGGCCA CGAGCTGCCA GTGCTGGCTC CCCAGTGGGA GGGCTACGAT GAGCTGCAGA 241 CTGATGGCAA CCGCAGCAGC CACTCCCGCT TGGGAAGAAT AGAGGCAGAT TCTGAAAGTC 301 AAGAAGACAT CATCCGGAAT ATTGCCAGGC ACCTCGCCCA GGTCGGGGAC AGCATGGACC 361 GTAGCATCCC TCCGGGCCTG GTGAACGGCC TGGCCCTGCA GCTCAGGAAC ACCAGCCGGT 421 CGGAGGAGGA CCGGAACAGG GACCTGGCCA CTGCCCTGGA GCAGCTGCTG CAGGCCTACC 481 CTAGAGACAT GGAGAAGGAG AAGACCATGC TGGTGCTGGC CCTGCTGCTG GCCAAGAAGG 541 TGGCCAGTCA AACGCCGTCC TTGCTCCGTG ATGTCTTTCA CACAACAGTG AACTTTATTA 601 ACCAGAACCT ACGCACCTAC GTGAGGAGCT TAGCCAGAAA TGGGATGGAC TGCCCAACTT 661 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 721 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 781 CTTGTGGAAA GGACGAACAA AGGCACGTTG ATGTCAATTG ACGCGTTAAG TCgacaatca 841 acctctggat tacaaaattt gtgaaagatt