Transcript: Human NM_198329.3

Homo sapiens ubiquitin like modifier activating enzyme 5 (UBA5), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-08-02
Taxon:
Homo sapiens (human)
Gene:
UBA5 (79876)
Length:
2865
CDS:
233..1279

Additional Resources:

NCBI RefSeq record:
NM_198329.3
NBCI Gene record:
UBA5 (79876)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_198329.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000422171 ACTTAGGGCAACATTAATTAA pLKO_005 1358 3UTR 100% 15.000 21.000 N UBA5 n/a
2 TRCN0000008016 GCACCGAACAAGGAACAGAAA pLKO.1 1977 3UTR 100% 4.950 6.930 N UBA5 n/a
3 TRCN0000008017 GCTTGTAATGAACTTGGACAA pLKO.1 644 CDS 100% 4.050 5.670 N UBA5 n/a
4 TRCN0000418046 CGTACCTTTGCCGTAGCAATA pLKO_005 278 CDS 100% 10.800 8.640 N UBA5 n/a
5 TRCN0000421863 CATACTCTGAGGAACATTAAT pLKO_005 461 CDS 100% 15.000 10.500 N UBA5 n/a
6 TRCN0000008018 CCTCAGTGTGATGACAGAAAT pLKO.1 965 CDS 100% 13.200 9.240 N UBA5 n/a
7 TRCN0000008019 GCAGGATTAAGTAAAGTTCAA pLKO.1 431 CDS 100% 4.950 3.465 N UBA5 n/a
8 TRCN0000011221 GCACTGCCTAAACAAGAGGTT pLKO.1 1025 CDS 100% 2.640 1.848 N UBA5 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_198329.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04142 pDONR223 100% 86.1% 86.1% None 0_1ins168 n/a
2 ccsbBroad304_04142 pLX_304 0% 86.1% 86.1% V5 0_1ins168 n/a
3 TRCN0000469518 GTCCTCTAGCTGACGGGCTATGTC pLX_317 30.3% 86.1% 86.1% V5 0_1ins168 n/a
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