Construct: ORF TRCN0000469518
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016930.1_s317c1
- Derived from:
- ccsbBroadEn_04142
- DNA Barcode:
- GTCCTCTAGCTGACGGGCTATGTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- UBA5 (79876)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469518
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79876 | UBA5 | ubiquitin like modifier act... | NM_024818.4 | 100% | 100% | |
| 2 | human | 79876 | UBA5 | ubiquitin like modifier act... | NM_001320210.1 | 86.1% | 86.1% | 0_1ins168 |
| 3 | human | 79876 | UBA5 | ubiquitin like modifier act... | NM_198329.3 | 86.1% | 86.1% | 0_1ins168 |
| 4 | human | 79876 | UBA5 | ubiquitin like modifier act... | NM_001321238.1 | 76.6% | 75.9% | (many diffs) |
| 5 | human | 79876 | UBA5 | ubiquitin like modifier act... | NM_001321239.1 | 72.2% | 72.2% | 0_1ins336 |
| 6 | human | 79876 | UBA5 | ubiquitin like modifier act... | XR_001740272.1 | 21.1% | 1_602del;1414_1415ins136;1679_4941del | |
| 7 | mouse | 66663 | Uba5 | ubiquitin-like modifier act... | NM_025692.3 | 85.2% | 87.6% | (many diffs) |
| 8 | mouse | 66663 | Uba5 | ubiquitin-like modifier act... | XM_006511791.2 | 62.1% | 65.1% | (many diffs) |
| 9 | mouse | 66663 | Uba5 | ubiquitin-like modifier act... | XR_001778969.1 | 40.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1278
- ORF length:
- 1212
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggagtctgtg gagcgcctgc agcagcgggt ccaggagctg gagcgggaac 121 ttgcccagga gaggagtctg caggtcccga ggagcggcga cggagggggc ggccgggtcc 181 gcatcgagaa gatgagctca gaggtggtgg attcgaatcc ctacagccgc ttgatggcat 241 tgaaacgaat gggaattgta agcgactatg agaaaatccg tacctttgcc gtagcaatag 301 taggtgttgg tggagtaggt agtgtgactg ctgaaatgct gacaagatgt ggcattggta 361 agttgctact ctttgattat gacaaggtgg aactagccaa tatgaataga cttttcttcc 421 aacctcatca agcaggatta agtaaagttc aagcagcaga acatactctg aggaacatta 481 atcctgatgt tctttttgaa gtacacaact ataatataac cacagtggaa aactttcaac 541 atttcatgga tagaataagt aatggtgggt tagaagaagg aaaacctgtt gatctagttc 601 ttagctgtgt ggacaatttt gaagctcgaa tgacaataaa tacagcttgt aatgaacttg 661 gacaaacatg gatggaatct ggggtcagtg aaaatgcagt ttcagggcat atacagctta 721 taattcctgg agaatctgct tgttttgcgt gtgctccacc acttgtagtt gctgcaaata 781 ttgatgaaaa gactctgaaa cgagaaggtg tttgtgcagc cagtcttcct accactatgg 841 gtgtggttgc tgggatctta gtacaaaacg tgttaaagtt tctgttaaat tttggtactg 901 ttagttttta ccttGGATAC AATGCAATGC AGGATTTTTT TCCTACTATG TCCATGAAGC 961 CAAATCCTCA GTGTGATGAC AGAAATTGCA GGAAGCAGCA GGAGGAATAT AAGAAAAAGG 1021 TAGCAGCACT GCCTAAACAA GAGGTTATAC AAGAAGAGGA AGAGATAATC CATGAAGATA 1081 ATGAATGGGG TATTGAGCTG GTATCTGAGG TTTCAGAAGA GGAACTGAAA AATTTTTCAG 1141 GTCCAGTTCC AGACTTACCT GAAGGAATTA CAGTGGCATA CACAATTCCA AAAAAGCAAG 1201 AAGATTCTGT CACTGAGTTA ACAGTGGAAG ATTCTGGTGA AAGCTTGGAA GACCTCATGG 1261 CCAAAATGAA GAATATGTAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1321 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1381 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAGTCCTCT AGCTGACGGG 1441 CTATGTCACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt