Transcript: Human NM_203463.3

Homo sapiens ceramide synthase 6 (CERS6), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-08-08
Taxon:
Homo sapiens (human)
Gene:
CERS6 (253782)
Length:
6804
CDS:
178..1332

Additional Resources:

NCBI RefSeq record:
NM_203463.3
NBCI Gene record:
CERS6 (253782)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_203463.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000344344 ACCACACGACTGGGTATATTT pLKO_005 994 CDS 100% 15.000 21.000 N CERS6 n/a
2 TRCN0000129261 CGGACGAACTAGGTGTTTAAT pLKO.1 1858 3UTR 100% 15.000 21.000 N CERS6 n/a
3 TRCN0000344343 CGGCTCATCTTCGAGAGATTT pLKO_005 331 CDS 100% 13.200 18.480 N CERS6 n/a
4 TRCN0000344264 ACTGACCTTCACTACTATTAC pLKO_005 703 CDS 100% 13.200 9.240 N CERS6 n/a
5 TRCN0000344345 CCCAAGCAAAGTGAACTATTT pLKO_005 1351 3UTR 100% 13.200 9.240 N CERS6 n/a
6 TRCN0000129817 CTCATCTTCGAGAGATTTGTA pLKO.1 334 CDS 100% 5.625 3.938 N CERS6 n/a
7 TRCN0000128836 GAACTGCTTCTGGTCTTACTT pLKO.1 1119 CDS 100% 5.625 3.938 N CERS6 n/a
8 TRCN0000344342 GAACTGCTTCTGGTCTTACTT pLKO_005 1119 CDS 100% 5.625 3.938 N CERS6 n/a
9 TRCN0000128857 GCTATTGCTAGTACAAGGGTT pLKO.1 1098 CDS 100% 2.640 1.848 N CERS6 n/a
10 TRCN0000128728 CCTGTTTGTTATGTTTGCCGT pLKO.1 963 CDS 100% 0.660 0.462 N CERS6 n/a
11 TRCN0000130926 CAGGCCAATGGACCACAAATT pLKO.1 385 CDS 100% 13.200 7.920 N CERS6 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_203463.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_13453 pDONR223 100% 97.9% 97.9% None 1002_1003ins24 n/a
2 ccsbBroad304_13453 pLX_304 0% 97.9% 97.9% V5 1002_1003ins24 n/a
3 TRCN0000472754 TGAAGACCTGGACACGGGATGATG pLX_317 38.1% 97.9% 97.9% V5 1002_1003ins24 n/a
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