Transcript: Mouse NM_212485.2

Mus musculus keratin 73 (Krt73), mRNA.

Source:
NCBI, updated 2017-05-28
Taxon:
Mus musculus (mouse)
Gene:
Krt73 (223915)
Length:
2154
CDS:
36..1655

Additional Resources:

NCBI RefSeq record:
NM_212485.2
NBCI Gene record:
Krt73 (223915)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_212485.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000090478 GCACCGTTGTATCTGGCATAA pLKO.1 2026 3UTR 100% 10.800 7.560 N Krt73 n/a
2 TRCN0000090480 GACTGGGTAGTGCAAGTGAAT pLKO.1 1573 CDS 100% 4.950 3.465 N Krt73 n/a
3 TRCN0000090481 GCATCTCCTTCAATGTGGCTA pLKO.1 199 CDS 100% 2.640 1.848 N Krt73 n/a
4 TRCN0000090482 CGCCCTGGATGGAGAGATCAA pLKO.1 806 CDS 100% 1.650 1.155 N Krt73 n/a
5 TRCN0000090479 CCACCAGGTAACAGTCAATAA pLKO.1 344 CDS 100% 13.200 7.920 N Krt73 n/a
6 TRCN0000108254 GCTTCAGCAGTCGGAGCCTTT pLKO.1 157 CDS 100% 1.350 0.945 N KRT73 n/a
7 TRCN0000116954 CCTCCTTCATCGACAAGGTAT pLKO.1 463 CDS 100% 4.950 2.475 Y KRT8P11 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_212485.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_13575 pDONR223 100% 59.9% 59% None (many diffs) n/a
2 ccsbBroad304_13575 pLX_304 0% 59.9% 59% V5 (many diffs) n/a
3 TRCN0000478787 CAGGTATTATTAGAGCATTGGAGC pLX_317 28% 59.9% 59% V5 (many diffs) n/a
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