Construct: ORF TRCN0000478787
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001340.1_s317c1
- Derived from:
- ccsbBroadEn_13575
- DNA Barcode:
- CAGGTATTATTAGAGCATTGGAGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KRT73 (319101)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478787
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 319101 | KRT73 | keratin 73 | NM_175068.3 | 69.5% | 68.3% | (many diffs) |
| 2 | human | 319101 | KRT73 | keratin 73 | XM_011538257.2 | 63.6% | 62.4% | (many diffs) |
| 3 | human | 112802 | KRT71 | keratin 71 | NM_033448.3 | 63.3% | 62.8% | (many diffs) |
| 4 | human | 112802 | KRT71 | keratin 71 | XM_017018749.1 | 49.1% | 48.9% | (many diffs) |
| 5 | human | 140807 | KRT72 | keratin 72 | XM_017018836.1 | 45.8% | 43.4% | (many diffs) |
| 6 | human | 140807 | KRT72 | keratin 72 | XM_017018835.1 | 45.2% | 36.4% | (many diffs) |
| 7 | human | 319101 | KRT73 | keratin 73 | XR_001748677.1 | 33.5% | (many diffs) | |
| 8 | mouse | 56735 | Krt71 | keratin 71 | NM_019956.1 | 61.2% | 61.3% | (many diffs) |
| 9 | mouse | 223915 | Krt73 | keratin 73 | NM_212485.2 | 59.9% | 59% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1212
- ORF length:
- 1143
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gagccgccaa ttcacctaca agtcgggacc tgctgccaag gggggcttca 121 gcggctgctc cgctgtgctc tcagggggca gctcatcctc ctaccgagca gggggcaaag 181 ggctcagtgg aggcttcagc agtcggagcc tttacagcct ggggggtgcc cggagcatct 241 ctttcaatgt ggccagtggc agtgggtggg caggaggcta tggatttggc cggggccggg 301 ccagtggctt tgctggcagc atgtttggca gtgtggcctt ggggtccgtg tgtccgtcgt 361 tgtgcccgcc cgggggtatc catcaggtca ccatcaacaa gagcctcctg gcacccctga 421 acgtggagct ggaccctgaa atccagaaag tgcgtgccca ggagcgggag cagatcaagg 481 tgctgaacaa caagttcgcc tccttcattg acaaggtgcg gttcctggag cagcagaacc 541 aggtgctgga gaccaagtgg gagctgctac agcagctgga cctgaacaac tgcaagaata 601 acctggagcc catccttgag ggctacatca gcaacctgcg gaagcagctg gagacgctgt 661 ctggggacag ggtgaggctg gactcggagc tgaggagcgt gcgcgaagtg gtggaggact 721 acaagaagag gtatgaagaa gaaataaaca agcgcacaac tgctgagaat gaatttgtgg 781 tgcttaagaa ggacgtggac gcagcttaca cgagcaaagt ggagctgcag gccaaggtgg 841 atgccctgga tggagaaatc aagttcttca agtgtctgta cgagggggag actgcTCAGA 901 TCCAGTCCCA CATCAGCGAC ACGTCCATCA TCCTGTCCAT GGACAACAAC CGGAACCTGG 961 ACCTGGACAG CATCATTGCT GAGGTCCGTG CCCAGTATGA GGAGATCGCC CGGAAGAGCA 1021 AGGCCGAGGC CGAGGCCCTG TACCAGACCA AGTTCCAGGA GCTGCAGCTA GCAGCCGGCC 1081 GGCATGGGGA TGACCTGAAA CACACCAAAA ATGAGATCTC AGAGCTGACC CGTCTCATCC 1141 AAAGACTGCG CTCGGAGATT GAGAGTGTGA AGAAGCAGGT GAAGGGGACT GGAGCCTTCC 1201 TTACTCACTC CTTGCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1261 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1321 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGACAG GTATTATTAG AGCATTGGAG 1381 CACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t