Transcript: Human NR_036647.1

Homo sapiens coiled-coil domain containing 144B (pseudogene) (CCDC144B), non-coding RNA.

Source:
NCBI, updated 2018-05-12
Taxon:
Homo sapiens (human)
Gene:
CCDC144B (284047)
Length:
8713
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
NR_036647.1
NBCI Gene record:
CCDC144B (284047)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NR_036647.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000257449 ACTTCGCAAGCCGACTTTAAT pLKO_005 3956 3UTR 100% 15.000 7.500 Y CCDC144A n/a
2 TRCN0000245928 ATCAGGTGACCTGCAATTATT pLKO_005 5474 3UTR 100% 15.000 7.500 Y CCDC144A n/a
3 TRCN0000245927 TATGAACCTGGATCCTATATA pLKO_005 4321 3UTR 100% 15.000 7.500 Y CCDC144A n/a
4 TRCN0000245926 AGAGGTCAGTACGCAACTTAC pLKO_005 4075 3UTR 100% 10.800 5.400 Y CCDC144A n/a
5 TRCN0000166971 GCCTGAAACCTAAATTAGAAA pLKO.1 1573 3UTR 100% 5.625 2.813 Y CCDC144B n/a
6 TRCN0000167035 CAAGGCAAATTAGAGTGGAAA pLKO.1 1014 3UTR 100% 4.950 2.475 Y CCDC144B n/a
7 TRCN0000141027 CACCTGAAAGTCTTCCTCAAA pLKO.1 553 3UTR 100% 4.950 2.475 Y CCDC144B n/a
8 TRCN0000139250 CCAGAAGTGGTTACGGTTGAA pLKO.1 1467 3UTR 100% 4.950 2.475 Y CCDC144B n/a
9 TRCN0000139683 CCTGGTTGTGAGGAAGAAGAT pLKO.1 1194 3UTR 100% 4.950 2.475 Y CCDC144B n/a
10 TRCN0000142491 GAACCCTAACTGATGGTACTA pLKO.1 1798 3UTR 100% 4.950 2.475 Y CCDC144B n/a
11 TRCN0000166942 GCAATGATGATGATGGACTAA pLKO.1 1825 3UTR 100% 4.950 2.475 Y CCDC144B n/a
12 TRCN0000168130 CCATCCATACTATCATCCGTA pLKO.1 1289 3UTR 100% 2.640 1.320 Y CCDC144B n/a
13 TRCN0000147095 CAGCAACTAGTTCCTGAATAT pLKO.1 519 3UTR 100% 0.000 0.000 Y CCDC144NL n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NR_036647.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_10599 pDONR223 100% 24.9% None 1_170del;2346_8713del n/a
2 ccsbBroad304_10599 pLX_304 0% 24.9% V5 1_170del;2346_8713del n/a
3 TRCN0000475733 GCAGCTCCCCGATGCTGGAGGGCG pLX_317 17% 24.9% V5 1_170del;2346_8713del n/a
4 ccsbBroadEn_05448 pDONR223 100% 6.5% None (many diffs) n/a
5 ccsbBroad304_05448 pLX_304 0% 6.5% V5 (many diffs) n/a
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