Transcript: Human NR_045925.2

Homo sapiens post-GPI attachment to proteins 2 (PGAP2), transcript variant 13, non-coding RNA.

Source:
NCBI, updated 2019-07-31
Taxon:
Homo sapiens (human)
Gene:
PGAP2 (27315)
Length:
1530
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
NR_045925.2
NBCI Gene record:
PGAP2 (27315)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NR_045925.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000425524 ACTTTCGGCACAACATGTATT pLKO_005 454 3UTR 100% 13.200 18.480 N PGAP2 n/a
2 TRCN0000426168 GTCCTCAAACATCACCTTTAC pLKO_005 811 3UTR 100% 10.800 8.640 N PGAP2 n/a
3 TRCN0000435086 TTCATAGGACTAATGTATTTC pLKO_005 1019 3UTR 100% 13.200 9.240 N PGAP2 n/a
4 TRCN0000412622 GAACAAGGAGCTGCTCATAAC pLKO_005 569 3UTR 100% 10.800 7.560 N PGAP2 n/a
5 TRCN0000061456 CACTGTTGTCTTAACCAACAT pLKO.1 515 3UTR 100% 0.495 0.347 N PGAP2 n/a
6 TRCN0000061453 CCATCCAGTTTCTGGCCTTTA pLKO.1 914 3UTR 100% 10.800 6.480 N PGAP2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NR_045925.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11863 pDONR223 100% 27.8% None 1_117del;282_283ins253;615_1530del n/a
2 ccsbBroad304_11863 pLX_304 0% 27.8% V5 1_117del;282_283ins253;615_1530del n/a
3 TRCN0000479879 CTTACCATCGGGAACAGGAACCTC pLX_317 46.6% 27.8% V5 1_117del;282_283ins253;615_1530del n/a
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