Transcript: Mouse NR_104378.1

Mus musculus F-box and leucine-rich repeat protein 4 (Fbxl4), transcript variant 2, non-coding RNA.

Source:
NCBI, updated 2015-02-15
Taxon:
Mus musculus (mouse)
Gene:
Fbxl4 (269514)
Length:
3702
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
NR_104378.1
NBCI Gene record:
Fbxl4 (269514)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NR_104378.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000086661 CGTCAGTTTAAACCTTGTATT pLKO.1 884 3UTR 100% 13.200 18.480 N Fbxl4 n/a
2 TRCN0000086660 CCCAAATCTACAAGACTTAAA pLKO.1 1519 3UTR 100% 13.200 10.560 N Fbxl4 n/a
3 TRCN0000086662 GCATCTAATTGTACCAGATTA pLKO.1 3121 3UTR 100% 13.200 9.240 N Fbxl4 n/a
4 TRCN0000086659 GCCCAAATCTACAAGACTTAA pLKO.1 1518 3UTR 100% 13.200 9.240 N Fbxl4 n/a
5 TRCN0000086658 GCTCTTCTGTTTGTTTGGTTT pLKO.1 3378 3UTR 100% 4.950 3.465 N Fbxl4 n/a
6 TRCN0000118341 GCCAGGACTATGTGGAACTTA pLKO.1 687 3UTR 100% 5.625 3.938 N FBXL4 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NR_104378.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02936 pDONR223 100% 44.6% None (many diffs) n/a
2 ccsbBroad304_02936 pLX_304 0% 44.6% V5 (many diffs) n/a
3 TRCN0000479278 ATAAATAATATTGACGATCGAGTG pLX_317 22.4% 44.6% V5 (many diffs) n/a
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