Transcript: Mouse NR_137170.1

Mus musculus coiled-coil domain containing 134 (Ccdc134), transcript variant 3, non-coding RNA.

Source:
NCBI, updated 2016-05-21
Taxon:
Mus musculus (mouse)
Gene:
Ccdc134 (76457)
Length:
1935
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
NR_137170.1
NBCI Gene record:
Ccdc134 (76457)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NR_137170.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000177633 GCCTTGGTTCTATTGAAATTA pLKO.1 1671 3UTR 100% 15.000 21.000 N Ccdc134 n/a
2 TRCN0000198720 CCCGAGCCTCAAGATTTACAA pLKO.1 178 3UTR 100% 5.625 7.875 N Ccdc134 n/a
3 TRCN0000217828 CCACTACTTTGACCATAATTC pLKO.1 387 3UTR 100% 13.200 9.240 N Ccdc134 n/a
4 TRCN0000173115 CATCCACCAGCAGTACAAGAT pLKO.1 265 3UTR 100% 4.950 3.465 N CCDC134 n/a
5 TRCN0000178031 GTACAAGATCCTGGATGTCAT pLKO.1 277 3UTR 100% 4.950 3.465 N Ccdc134 n/a
6 TRCN0000181668 GAATGACATCCACCAGCAGTA pLKO.1 259 3UTR 100% 4.050 2.835 N Ccdc134 n/a
7 TRCN0000198685 CAGCAGTACAAGATCCTGGAT pLKO.1 272 3UTR 100% 2.640 1.848 N Ccdc134 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NR_137170.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04144 pDONR223 100% 26% None (many diffs) n/a
2 ccsbBroad304_04144 pLX_304 0% 26% V5 (many diffs) n/a
3 TRCN0000471535 GCTGATATCACACAGGAATAAGCG pLX_317 50.7% 26% V5 (many diffs) n/a
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