Transcript: Human NR_144546.1

Homo sapiens ubiquitin B pseudogene 4 (UBBP4), non-coding RNA.

Source:
NCBI, updated 2018-12-05
Taxon:
Homo sapiens (human)
Gene:
UBBP4 (23666)
Length:
1475
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
NR_144546.1
NBCI Gene record:
UBBP4 (23666)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NR_144546.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000413456 GAAGATGGCCGCACTCTTTCT pLKO_005 1231 3UTR 100% 4.950 2.475 Y UBB n/a
2 TRCN0000011103 GTGAAGGCCAAGATCCAAGAT pLKO.1 701 3UTR 100% 4.950 2.475 Y UBB n/a
3 TRCN0000011102 CCTGCGTCTGAGAGGTGGTAT pLKO.1 835 3UTR 100% 1.650 0.825 Y UBB n/a
4 TRCN0000414438 TGAAGACCCTGACCGGCAAGA pLKO_005 639 3UTR 100% 1.350 0.675 Y UBB n/a
5 TRCN0000007736 CCGCACTCTTTCTGACTACAA pLKO.1 1239 3UTR 100% 4.950 2.475 Y UBB n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NR_144546.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14873 pDONR223 70.1% 44.9% None (many diffs) n/a
2 ccsbBroad304_14873 pLX_304 0% 44.9% V5 (many diffs) n/a
3 ccsbBroadEn_14872 pDONR223 58.4% 44.2% None (many diffs) n/a
4 ccsbBroad304_14872 pLX_304 0% 44.2% V5 (many diffs) n/a
5 TRCN0000468005 TCGGGCCTGTCTTATCTTATATCC pLX_317 100% 14.6% V5 (many diffs) n/a
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