Construct: ORF ccsbBroad304_14872
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004019.1_s304c1, BRDN0000402863
- Derived from:
- ccsbBroadEn_14872
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- UBB (7314)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14872
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7314 | UBB | ubiquitin B | NM_001281716.1 | 98.6% | 100% | (many diffs) |
| 2 | human | 7314 | UBB | ubiquitin B | NM_001281717.1 | 98.6% | 100% | (many diffs) |
| 3 | human | 7314 | UBB | ubiquitin B | NM_001281718.1 | 98.6% | 100% | (many diffs) |
| 4 | human | 7314 | UBB | ubiquitin B | NM_001281719.1 | 98.6% | 100% | (many diffs) |
| 5 | human | 7314 | UBB | ubiquitin B | NM_001281720.1 | 98.6% | 100% | (many diffs) |
| 6 | human | 7314 | UBB | ubiquitin B | NM_018955.4 | 98.6% | 100% | (many diffs) |
| 7 | human | 23666 | UBBP4 | ubiquitin B pseudogene 4 | NR_144546.1 | 44.2% | (many diffs) | |
| 8 | human | 7311 | UBA52 | ubiquitin A-52 residue ribo... | NM_001033930.3 | 40.7% | 27.1% | (many diffs) |
| 9 | human | 7311 | UBA52 | ubiquitin A-52 residue ribo... | NM_001321018.2 | 40.7% | 27.1% | (many diffs) |
| 10 | human | 7311 | UBA52 | ubiquitin A-52 residue ribo... | NM_001321019.2 | 40.7% | 27.1% | (many diffs) |
| 11 | human | 7311 | UBA52 | ubiquitin A-52 residue ribo... | NM_001321020.2 | 40.7% | 27.1% | (many diffs) |
| 12 | human | 7311 | UBA52 | ubiquitin A-52 residue ribo... | NM_003333.5 | 40.7% | 27.1% | (many diffs) |
| 13 | human | 7316 | UBC | ubiquitin C | NM_021009.7 | 29.1% | 33.2% | (many diffs) |
| 14 | mouse | 22187 | Ubb | ubiquitin B | NM_001313984.1 | 69% | 74.7% | (many diffs) |
| 15 | mouse | 22187 | Ubb | ubiquitin B | NM_011664.4 | 69% | 74.7% | (many diffs) |
| 16 | mouse | 218963 | Gm1821 | predicted gene 1821; ubiqui... | NR_002875.2 | 63.7% | (many diffs) | |
| 17 | mouse | 22186 | Uba52 | ubiquitin A-52 residue ribo... | NM_001348227.1 | 41.9% | 27.1% | (many diffs) |
| 18 | mouse | 22186 | Uba52 | ubiquitin A-52 residue ribo... | NM_001348228.1 | 41.9% | 27.1% | (many diffs) |
| 19 | mouse | 22186 | Uba52 | ubiquitin A-52 residue ribo... | NM_001348229.1 | 41.9% | 27.1% | (many diffs) |
| 20 | mouse | 22186 | Uba52 | ubiquitin A-52 residue ribo... | NM_019883.4 | 41.9% | 27.1% | (many diffs) |
| 21 | mouse | 22190 | Ubc | ubiquitin C | NM_019639.4 | 27.9% | 31% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 753
- ORF length:
- 687
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgca gatcttcgtg aaaaccctta ccggcaagac catcaccctt gaggtggagc 121 ccagtgacac catcgaaaat gtgaaggcca agatccagga taaggaaggc attccccccg 181 accagcagag gctcatcttt gcaggcaagc agctggaaga cggccgtact ctttctgact 241 acaacatcca gaaggagtcg accctgcacc tggtcctgcg cctgaggggt ggtatgcaga 301 tcttcgtgaa gaccctgacc ggcaagacca tcaccctgga agtggagccc agtgacacca 361 tcgaaaatgt gaaggccaag atccaggata aagaaggcat ccctcccgac cagcagaggc 421 tcatctttgc aggcaagcag ctggaagatg gccgcactct ttctgactac aacatccaga 481 aggagtcgac cctgcacctg gtcctgcgtc tgagaggtgg tatgcagatc ttcgtgaaga 541 ccctgaccgg caagaccatc actctggagg tggagcccag tgacaccatc gaaaatgtga 601 aggccaagat ccaggataag gaaggcattc cccccgacca gcagaggctc atctttgcag 661 gcaagcagct ggaagacggc cgtactcttt ctgactacaa catccagaag gagtcgaccc 721 tgcacctggt cctgcgcctg aggggtggct gttacccaac tttcttgtac aaagtggttg 781 gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga gctagcgcta 841 accggtggcg cgttaagtcg acaatcaacc tctggatta